Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Histone H2A.Z抗体[4A4] (ab80150)

Reviews (2)Q&A (2)
Western blot - Anti-Histone H2A.Z antibody [4A4] (ab80150)

    Key features and details

    • Rat monoclonal [4A4] to Histone H2A.Z
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG2b

    概述

    • 产品名称

      Anti-Histone H2A.Z抗体[4A4]
      参阅全部 Histone H2A.Z 一抗

    • 描述

      大鼠单克隆抗体[4A4] to Histone H2A.Z

    • 宿主

      Rat

    • 经测试应用

      适用于: WBmore details
      不适用于: Flow Cyt,ICC/IF or IHC-P

    • 种属反应性

      与反应: Recombinant fragment
      预测可用于: Mouse, Rat, Cow, Human, Pig, Xenopus laevis, Zebrafish, Orangutan

    • 免疫原

      Synthetic peptide corresponding to Human Histone H2A.Z.

    • 阳性对照

      • This antibody gave a positive signal when used to probe Histone H2A.Z recombinant protein.

    • 常规说明

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 纯度

      IgG fraction

    • 克隆

      单克隆

    • 克隆编号

      4A4

    • 同种型

      IgG2b

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab80150于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
    说明
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
    应用说明
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    靶标

    • 功能

      Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

    • 序列相似性

      Belongs to the histone H2A family.

    • 翻译后修饰

      Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
      Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
      Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
      Not phosphorylated.

    • 细胞定位

      Nucleus. Chromosome.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • H2A histone family member Z antibody
      • H2A.z antibody
      • H2A/z antibody
      • H2afz antibody
      • H2AZ antibody
      • H2AZ_HUMAN antibody
      • Histone H2A.Z antibody
      • MGC117173 antibody
      see all

    图片

    • Western blot - Anti-Histone H2A.Z antibody [4A4] (ab80150)
      Western blot - Anti-Histone H2A.Z antibody [4A4] (ab80150)
      All lanes : Anti-Histone H2A.Z antibody [4A4] (ab80150) at 1 µg/ml

      Lane 1 : Histone H2A.Z recombinant protein
      Lane 2 : Histone H2A recombinant protein

      Lysates/proteins at 0.5 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

      Predicted band size: 13 kDa
      Observed band size: 17 kDa why is the actual band size different from the predicted?
      Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

    文献 (0)

    发表研究结果有使用 ab80150？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab80150 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2A.Z antibody [4A4]

     Poor
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    提交于 Mar 29 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2A.Z antibody [4A4]

     Average
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreatic cancer)
    Specification
    Pancreatic cancer
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Antibody Solutions

    Verified customer

    提交于 Mar 11 2010

    Question

    This is regarding the collaboration discount bellow. It looks like the antibody we were supposed to test has now been removed from the Abcam catalogue, making it difficult for us to submit an abreview…

    We have now tested the antibody in western, but are not too sure how to proceed further and how to activate our discount code.

    Thanks for your help,

    Best wishes,

    Read More
    Answer

    Thank you for contacting me.

    I am sorry that the Anti-Histone H2A.Z antibody (ab97966) has now been removed from the catalogue. I am sorry but I was not aware of this change when I suggested you test it. Let me reassure you that the antibody has not been removed due to any quality issues (if this were the case anyone who had purchased the affected antibody would have been informed).

    You should still be able to submit an Abreview for the product. This can be done by following the link below:

    https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews

    And clicking on the "https://www.abcam.com/index.html?pageconfig=your_reviews&strAccessMode=public_create".

    You will need to log in to your account in order to achieve this. If you have any problems submitting the review please do let me know. As soon as the Abreview has been submitted, the discount code can be used to claim your free primary antibody (or the equivalent price of ab97966 off any product).

    I hope this information has been of help. If you have any further questions please do let me know.

    Read More

    Question


    Thanks for your help with that order. I have another question for an additional project we are initiating requiring lots of different antibodies. This is a clinical diagnostics projects, and all antibodies we will use for that need to be CE certified for diagnostic purposes. Since I did not find any information on this on your website, I wanted to ask you if you have any CE certified antibodies directed against these (human) proteins:

    Read More
    Answer

    Thank you for contacting Abcam.

    All of our products are for research purposes only and are not certified for clinical research applications.

    I am sorry that I could not be more helpful in this matter.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com