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AB11175

Anti-Histone H2A.X 抗体

Anti-Histone H2A.X antibody

5

(43 Reviews)

|

(159 Publications)

Anti-Histone H2A.X antibody (ab11175) is a rabbit polyclonal antibody detecting Histone H2A.X in Western Blot, IHC-P. Suitable for Human, Mouse.

- Over 150 publications
- Trusted since 2004

查看别名

H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X

3 Images
Western blot - Anti-Histone H2A.X antibody (AB11175)
  • WB

Supplier Data

Western blot - Anti-Histone H2A.X antibody (AB11175)

Detection : Chemiluminescence

All lanes:

Western blot - Anti-Histone H2A.X antibody (ab11175) at 0.1 µg/mL

Lane 1:

F9 cell lysate (prepared using RIPA lysis buffer) at 50 µg

Lane 2:

L1210 cell lysate (prepared using RIPA lysis buffer) at 50 µg

Predicted band size: 15 kDa

false

Exposure time: 30s

Western blot - Anti-Histone H2A.X antibody (AB11175)
  • WB

Unknown

Western blot - Anti-Histone H2A.X antibody (AB11175)

All lanes:

Western blot - Anti-Histone H2A.X antibody (ab11175) at 0.1 µg/mL

All lanes:

HEK293T cells at 50 µg

Predicted band size: 15 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB11175)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (AB11175)

mmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling H2A.X with ab11175 at 1 μg/mL.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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您是否考虑以下替代产品？

AB124781

Anti-Histone H2A.X antibody [EPR895] - Nuclear Marker

5
5 Reviews
查看产品
为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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产品详情

What is this antibody validated in?
Anti-Histone H2A.X antibody (ab11175) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

Trusted by the scientific community
Anti-Histone H2A.X (ab11175) was first used in a scientific publication in 2004 and has been cited over 150 times in peer-reviewed journals.

Reviewed by scientists
Anti-Histone H2A.X (ab11175) has over 40 independent reviews from customers.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Antibodies were affinity purified using the peptide immobilized on solid support.
存储溶液
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: Tris citrate/phosphate
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

The protein expressed by the H2AX gene is a variant histone H2A that replaces conventional H2A in certain nucleosomes, which are responsible for wrapping and compacting DNA into chromatin. This compaction limits DNA accessibility to cellular machineries that require DNA as a template, placing histones at the center of transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is controlled through a complex array of post-translational histone modifications, known as the histone code, and nucleosome remodeling. The H2AX protein is essential for the checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for the efficient repair of DNA double strand breaks (DSBs), particularly when it undergoes C-terminal phosphorylation. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H2AX
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文献 (159)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37175793

2023

Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia-Reperfusion-Induced Acute Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jelena Nesovic Ostojic,Maja Zivotic,Sanjin Kovacevic,Milan Ivanov,Predrag Brkic,Nevena Mihailovic-Stanojevic,Danijela Karanovic,Una Jovana Vajic,Zoran Miloradovic,Djurdjica Jovovic,Sanja Radojevic Skodric

Oncology reports 49: PubMed36734271

2023

AZD6738 promotes the tumor suppressive effects of trifluridine in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shinnosuke Harata,Takuya Suzuki,Hiroki Takahashi,Takahisa Hirokawa,Akira Kato,Kaori Watanabe,Takeshi Yanagita,Hajime Ushigome,Kazuyoshi Shiga,Ryo Ogawa,Akira Mitsui,Masahiro Kimura,Yoichi Matsuo,Shuji Takiguchi

Molecular and cellular biology 42:e0037922 PubMed36278823

2022

Impact of Nuclear NAD Synthesis via Histone Dynamics on DNA Repair during Cellular Senescence To Prevent Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Masae Ikura,Kanji Furuya,Tomonari Matsuda,Tsuyoshi Ikura

Scientific reports 12:17729 PubMed36273072

2022

Noxa and Mcl-1 expression influence the sensitivity to BH3-mimetics that target Bcl-xL in patient-derived glioma stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana Belén Vera,Olivia Morris-Hanon,Germán Ignacio Nogueiras,Luisina Belén Ripari,Myrian Inés Esquivel,Carolina Perez-Castro,Leonardo Romorini,Gustavo Emilio Sevlever,María Elida Scassa,Guillermo Agustín Videla-Richardson

Molecular cell 82:3794-3809.e8 PubMed36206766

2022

Calcineurin dephosphorylates topoisomerase IIβ and regulates the formation of neuronal-activity-induced DNA breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Ilse Delint-Ramirez,Lahiri Konada,Lance Heady,Richard Rueda,Alvaro Sebastian Vaca Jacome,Eric Marlin,Charlotte Marchioni,Amir Segev,Oleg Kritskiy,Satoko Yamakawa,Andrew H Reiter,Li-Huei Tsai,Ram Madabhushi

Journal of medical genetics 59:1227-1233 PubMed36041817

2022

Loss of seryl-tRNA synthetase () causes complex spastic paraplegia and cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Edgard Verdura,Bruno Senger,Miquel Raspall-Chaure,Agatha Schlüter,Nathalie Launay,Montserrat Ruiz,Carlos Casasnovas,Agustí Rodriguez-Palmero,Alfons Macaya,Hubert Dominique Becker,Aurora Pujol

iScience 25:104944 PubMed36065181

2022

Phosphorylation of BRCA1 by ATM upon double-strand breaks impacts ATM function in end-resection: A potential feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Qi,Reka Chakravarthy,Monica M Li,Chu-Xia Deng,Rong Li,Yanfen Hu

Life science alliance 5: PubMed35905994

2022

RFWD3 and translesion DNA polymerases contribute to PCNA modification-dependent DNA damage tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Rie Kanao,Hidehiko Kawai,Toshiyasu Taniguchi,Minoru Takata,Chikahide Masutani

Oncology reports 47: PubMed35191521

2022

ATR inhibitor AZD6738 increases the sensitivity of colorectal cancer cells to 5‑fluorouracil by inhibiting repair of DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Suzuki,Takahisa Hirokawa,Anri Maeda,Shinnosuke Harata,Kaori Watanabe,Takeshi Yanagita,Hajime Ushigome,Nozomi Nakai,Yuzo Maeda,Kazuyoshi Shiga,Ryo Ogawa,Akira Mitsui,Masahiro Kimura,Yoichi Matsuo,Hiroki Takahashi,Shuji Takiguchi

Protein & cell 13:580-601 PubMed35147915

2022

BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhu Wang,Kun Zhao,Meng Liu,Mengting Wang,Zhibin Qiao,Shanru Yi,Yonghua Jiang,Xiaochen Kou,Yanhong Zhao,Jiqing Yin,Tianming Li,Hong Wang,Cizhong Jiang,Shaorong Gao,Jiayu Chen
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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