AB222765

重组Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1)抗体[RM216]

Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216]

Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Histone H4 phospho S1 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human H4C1 phospho S1.

查看别名

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4S1p

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (AB222765)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (AB222765)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H2A (phospho S1) + Histone H4 (phospho S1) (red) using ab222765 at 2 μg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).

ELISA - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (AB222765)

ELISA

Supplier Data

ELISA - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (AB222765)

ab222765 specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).``

Supplier Data

Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (AB222765)

All lanes:

Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765) at 0.5 µg/mL

Lane 1:

Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Nocodazole

Lane 2:

Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells non-treated with Nocodazole

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关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

RM216

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, WB, ELISA

applications

免疫原

Synthetic Peptide within Human H4C1 phospho S1. The exact immunogen used to generate this antibody is proprietary information.

P62805

特异性

ab222765 reacts to Histone H4 or Histone H2A phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "" }, "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

See full target information H4C1 phospho S1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 14: PubMed39334865

2024

Biochemical Characterisation of the Short Isoform of Histone N-Terminal Acetyltransferase NAA40.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel Klavaris,Maria Kouma,Cem Ozdemir,Vicky Nicolaidou,Kyle M Miller,Costas Koufaris,Antonis Kirmizis

Nature communications 15:7694 PubMed39227587

2024

Phosphorylation activates master growth regulator DELLA by promoting histone H2A binding at chromatin in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Huang,Rodolfo Zentella,Jeongmoo Park,Larry Reser,Dina L Bai,Mark M Ross,Jeffrey Shabanowitz,Donald F Hunt,Tai-Ping Sun

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1)抗体[RM216]

Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216]

Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (AB222765)

ELISA - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (AB222765)

Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (AB222765)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性