重组Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1)抗体[RM216] (ab222765)
![Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)](https://www.abcam.cn/ps/products/222/ab222765/Images/ab222765-282532-anti-histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-rm216-western-blot.jpg "Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM216] to Histone H2A (phospho S1) + Histone H4 (phospho S1)
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human
概述
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产品名称
Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1)抗体[RM216] -
描述
兔单克隆抗体[RM216] to Histone H2A (phospho S1) + Histone H4 (phospho S1) -
宿主
Rabbit -
特异性
ab222765 reacts to Histone H4 or Histone H2A phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones. -
经测试应用
适用于: ICC/IF, WB, ELISAmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Histone H2A (phospho S1).
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阳性对照
- WB: Acid extract of HeLa cells treated with Nocodazole. ICC/IF: HeLa cells.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
RM216 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab222765于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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| WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14, 11 kDa.
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| ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 - 2 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14, 11 kDa. |
|
ELISA
Use at an assay dependent concentration. |
靶标
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细胞定位
Histone H2A: Nucleus. Chromosome. Histone H4: Nucleus. Chromosome. -
数据库链接
- Entrez Gene: 121504 Human
- Entrez Gene: 3012 Human
- Entrez Gene: 317772 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8335 Human
- Entrez Gene: 8337 Human
- Entrez Gene: 8338 Human
see all
图片
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Western blot - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)All lanes : Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765) at 0.5 µg/ml
Lane 1 : Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Nocodazole
Lane 2 : Acid extract of HeLa (human epithelial cell line from cervix adenocarcinoma) cells non-treated with Nocodazole
Predicted band size: 14, 11 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)](https://www.abcam.cn/ps/products/222/ab222765/Images/ab222765-282533-anti-histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-rm216-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H2A (phospho S1) + Histone H4 (phospho S1) (red) using ab222765 at 2 µg/ml in ICC/IF. Actin filaments have been labeled with fluorescein phalloidin (green).
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![ELISA - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)](https://www.abcam.cn/ps/products/222/ab222765/Images/ab222765-282531-anti-histone-h2a-phospho-s1-histone-h4-phospho-s1-antibody-rm216.jpg)
ELISA - Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)ab222765 specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p).
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![Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)](https://www.abcam.cn/ps/products/222/ab222765/Images/ab222765-4-benefits-of-recombinant-antibodies.png)
Anti-Histone H2A (phospho S1) + Histone H4 (phospho S1) antibody [RM216] (ab222765)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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