Histone H2A+Histone H4重组抗体[EPR16995]_Histone H2A+Histone H4抗体(ab177186)

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Western blot - Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] (ab177186)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16995] to Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3)
Suitable for: WB, ICC/IF, IHC-P, PepArr
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3)抗体[EPR16995]
参阅全部 Histone H2A+Histone H4 一抗
描述

兔单克隆抗体[EPR16995] to Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3)
宿主

Rabbit
经测试应用

适用于: WB, ICC/IF, IHC-P, PepArrmore details
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HeLa and NIH/3T3 cell lysates. IHC-P: Human, mouse and rat colon tissues. ICC/IF: HeLa cells.
常规说明

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR16995
同种型

IgG
研究领域

Alternative Versions
- Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] - BSA and Azide (ab271927)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise™承诺保证使用ab177186于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11, 14 kDa).
ICC/IF		1/2000.
IHC-P		1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
PepArr		Use a concentration of 0.025 µg/ml.

说明
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11, 14 kDa).
ICC/IF 1/2000.
IHC-P 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
PepArr Use a concentration of 0.025 µg/ml.

细胞定位

Histone H2A: Nucleus. Chromosome. Histone H4: Nucleus. Chromosome.
数据库链接
- Entrez Gene: 121504 Human
- Entrez Gene: 3012 Human
- Entrez Gene: 317772 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8335 Human
- Entrez Gene: 8337 Human
- Entrez Gene: 8338 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
- Entrez Gene: 8364 Human
- Entrez Gene: 8365 Human
- Entrez Gene: 8366 Human
- Entrez Gene: 8367 Human
- Entrez Gene: 8368 Human
- Entrez Gene: 8370 Human
- Entrez Gene: 100041230 Mouse
- Entrez Gene: 100862646 Mouse
- Entrez Gene: 319155 Mouse
- Entrez Gene: 319156 Mouse
- Entrez Gene: 319157 Mouse
- Entrez Gene: 319158 Mouse
- Entrez Gene: 319159 Mouse
- Entrez Gene: 319160 Mouse
- Entrez Gene: 319161 Mouse
- Entrez Gene: 319166 Mouse
- Entrez Gene: 320332 Mouse
- Entrez Gene: 326619 Mouse
- Entrez Gene: 326620 Mouse
- Entrez Gene: 69386 Mouse
- Entrez Gene: 97122 Mouse
- Entrez Gene: 64646 Rat
- Omim: 142720 Human
- Omim: 142750 Human
- Omim: 602786 Human
- Omim: 602797 Human
- Omim: 602822 Human
- Omim: 602823 Human
- Omim: 602824 Human
- Omim: 602825 Human
- Omim: 602826 Human
- Omim: 602827 Human
- Omim: 602828 Human
- Omim: 602829 Human
- Omim: 602830 Human
- Omim: 602831 Human
- Omim: 602833 Human
- SwissProt: P04908 Human
- SwissProt: P28001 Human
- SwissProt: P62805 Human
- SwissProt: Q6DRA9 Human
- SwissProt: Q93077 Human
- SwissProt: Q99878 Human
- SwissProt: P10812 Mouse
- SwissProt: P22752 Mouse
- SwissProt: P62806 Mouse
- SwissProt: P02262 Rat
- Unigene: 121017 Human
- Unigene: 143080 Human
- Unigene: 247816 Human
- Unigene: 248172 Human
- Unigene: 248174 Human
- Unigene: 248178 Human
- Unigene: 248179 Human
- Unigene: 278483 Human
- Unigene: 46423 Human
- Unigene: 528055 Human
- Unigene: 533295 Human
- Unigene: 55468 Human
- Unigene: 591790 Human
- Unigene: 655235 Human
- Unigene: 662174 Human
- Unigene: 706635 Human
- Unigene: 742244 Human
- Unigene: 14775 Mouse
- Unigene: 158272 Mouse
- Unigene: 227295 Mouse
- Unigene: 228709 Mouse
- Unigene: 246720 Mouse
- Unigene: 255646 Mouse
- Unigene: 260530 Mouse
- Unigene: 261642 Mouse
- Unigene: 261662 Mouse
- Unigene: 261664 Mouse
- Unigene: 377875 Mouse
- Unigene: 442307 Mouse
- Unigene: 486099 Mouse
- Unigene: 489077 Mouse
see all

Western blot - Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] (ab177186)

Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] (ab177186) at 1/1000 dilution + HeLa (human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 11, 14 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] (ab177186)

Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] (ab177186) at 1/1000 dilution + NIH/3T3 (mouse embyro fibroblast cells) cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 11, 14 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Peptide Array - Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] (ab177186)

ab177186 was tested in Peptide Array against human synthetic peptides to different Histone modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to histone H2A R3me1peptide (ab28756) and H4 R3me1peptide(ab17770), indicating that this antibody specifically recognises the histone H2A R3me1peptide (ab28756) and H4 R3me1peptide(ab17770).

ab28756 - H2A R3me1
ab17770 - H4 R3me1
ab178994 - H2A unmod(1-30)
ab179297 - H4 unmod(1-30)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] (ab177186)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) with ab177186 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human colon tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] (ab177186)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) with ab177186 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on mouse colon tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] (ab177186)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) with ab177186 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on rat colon tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] (ab177186)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) with ab177186 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab177186 at 1/4000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Anti-Histone H2A (mono methyl R3) + Histone H4 (mono methyl R3) antibody [EPR16995] (ab177186)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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发表研究结果有使用 ab177186？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab177186 被引用在 1 文献中.

Bhagwani AR et al. A p53-TLR3 axis ameliorates pulmonary hypertension by inducing BMPR2 via IRF3. iScience 26:105935 (2023). PubMed: 36685041

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

应用

The Abpromise guarantee

靶标

细胞定位

数据库链接

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (1)

客户评价及客户问答