Anti-Histone H2A-Bbd抗体
Anti-Histone H2A-Bbd antibody
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(3 Publications)
Rabbit Polyclonal H2A.B antibody. Suitable for ICC, WB and reacts with Mouse, Chicken, Human samples. Cited in 3 publications.
查看别名
H2AFB2, H2AB3, H2ABBD, H2AFB, H2AFB3, H2AB2, Histone H2A-Bbd type 2/3, H2A Barr body-deficient, H2A.Bbd
- ICC
Collaborator
Immunocytochemistry - Anti-Histone H2A-Bbd antibody (AB4175)
Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with ab4175 (anti-H2A.Bbd) antibody o/n. The antibody appears to show nucleolar staining. The antibody gave the same staining pattern in different cell types. The dilution used was 3ug/ml.
- WB
Supplier Data
Western blot - Anti-Histone H2A-Bbd antibody (AB4175)
H2A-Bbd (ab4175)
CE= chicken erythrocyte (10 and 2 micrograms) , HE= HeLa cells (10 micrograms) and 2A*= recombinant H2ABbd (the numbers underneath the lanes are the number of nanograms loaded in each lane).
The primary antibody dilution was 1 : 500 and the secondary was 1 : 3000.
As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.
All lanes:
Western blot - Anti-Histone H2A-Bbd antibody (ab4175)
Predicted band size: 13 kDa
false
Juan Ausio
关键信息
宿主种属
Rabbit
克隆
Polyclonal
亚型
IgG
不含载体蛋白
No
反应种属
Mouse, Chicken, Human
应用
WB, ICC
免疫原
The exact immunogen used to generate this antibody is proprietary information.
特异性
Known to detect the recombinant form of human Histone H2A BBD. However, no reaction has yet been demonstrated against the endogenous protein in histone preparations. We believe that this is because of the low abundance of this variant in such preparations.
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反应性数据
{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p>As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.</p>" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "3 µg/mL", "ICC-species-notes": "<p>Staining may not be specific for H2A.Bbd (see image).</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p>As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.</p>" } } }
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性能和储存信息
形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
运输条件
Blue Ice
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案
For this product, it's our understanding that no specific protocols are required. You can visit:
- Visit the General protocols
- Visit the Troubleshooting
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靶点信息
Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. They associate with spliceosome components and are required for mRNA splicing. May participate in spermatogenesis.
See full target information H2AB2
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文献 (3)
Recent publications for all applications. Explore the full list and refine your search
Genetics and molecular research : GMR 13:5929-39 PubMed25117351
2014
Localization and expression of histone H2A variants during mouse oogenesis and preimplantation embryo development.
Applications
Unspecified application
Species
Unspecified reactive species
B J Wu,F L Dong,X S Ma,X G Wang,F Lin,H L Liu
Development (Cambridge, England) 137:3785-94 PubMed20943707
2010
Changes in the nuclear deposition of histone H2A variants during pre-implantation development in mice.
Applications
WB, ICC/IF
Species
Mouse, Mouse
Buhe Nashun,Masashi Yukawa,Honglin Liu,Tomohiko Akiyama,Fugaku Aoki
Human molecular genetics 18:2567-74 PubMed19380460
2009
Chromatin analysis of occluded genes.
Applications
ChIP
Species
Human
Jae Hyun Lee,Jedidiah Gaetz,Branimir Bugarija,Croydon J Fernandes,Gregory E Snyder,Eliot C Bush,Bruce T Lahn
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