重组Anti-Histone H2A (acetyl K5)抗体[EP856Y] (ab45152)
![Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)](https://www.abcam.cn/ps/products/45/ab45152/Images/ab45152-213846-anti-histone-h2a-acetyl-k5-antibody-ep856y-western-blot.jpg "Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP856Y] to Histone H2A (acetyl K5)
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Histone H2A (acetyl K5)抗体[EP856Y]
参阅全部 Histone H2A 一抗 -
描述
兔单克隆抗体[EP856Y] to Histone H2A (acetyl K5) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Caenorhabditis elegans
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human breast carcinoma tissue and C6 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP856Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45152于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (7) |
1/100000. Predicted molecular weight: 14 kDa.
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| IHC-P | (2) |
1/250 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF | (3) |
1/200 - 1/1000.
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| IP |
1/20.
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| 说明 |
|---|
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WB
1/100000. Predicted molecular weight: 14 kDa. |
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IHC-P
1/250 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/200 - 1/1000. |
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IP
1/20. |
靶标
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功能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
序列相似性
Belongs to the histone H2A family. -
翻译后修饰
The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
Deiminated on Arg-4 in granulocytes upon calcium entry.
Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 191549 Caenorhabditis elegans
- Entrez Gene: 3012 Human
- Entrez Gene: 317772 Human
- Entrez Gene: 8335 Human
- Entrez Gene: 8338 Human
- Entrez Gene: 319166 Mouse
- Entrez Gene: 64646 Rat
- Omim: 142720 Human
see all -
别名
- H2a 615 antibody
- H2A antibody
- H2A GL101 antibody
see all
图片
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Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)This image is courtesy of an anonymous AbreviewAnti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/1000 dilution (unpurified) + Mouse cortex whole tissue lysate at 20 µg
Secondary
HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)](https://www.abcam.cn/ps/products/45/ab45152/Images/ab45152-259512-anti-histone-h2a-acetyl-k5-antibody-ep856y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)ab45152 staining Histone H2A (acetyl K5) in C6 (rat glioma) cells, treated and untreated with TSA,500ng/ml for 4 hours by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab75748 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) -
![Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)](https://www.abcam.cn/ps/products/45/ab45152/Images/ab45152-259511-anti-histone-h2a-acetyl-k5-antibody-ep856y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)ab45152 immunoprecipitating Histone H2A. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.
Lane 1: Mouse brain whole cell lysate
Lane 2: Mouse brain whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab45152 in Mouse brain whole cell lysate -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)](https://www.abcam.cn/ps/products/45/ab45152/Images/ab45152-259522-anti-histone-h2a-acetyl-k5-antibody-ep856y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)ab45152 staining Histone H2A (acetyl K5) in rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)](https://www.abcam.cn/ps/products/45/ab45152/Images/ab45152-259518-anti-histone-h2a-acetyl-k5-antibody-ep856y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)ab45152 staining Histone H2A (acetyl K5) in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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![Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)](https://www.abcam.cn/ps/products/45/ab45152/Images/ab45152-57319-anti-histone-h2a-acetyl-k5-antibody-ep856y-western-blot.jpg)
Western blot - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)All lanes : Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/100000 dilution (unpurified)
Lane 1 : C6 cell lysate
Lane 2 : C6 + TSA membrane
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)](https://www.abcam.cn/ps/products/45/ab45152/Images/ab45152-259515-anti-histone-h2a-acetyl-k5-antibody-ep856y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)ab45152 staining Histone H2A (acetyl K5) in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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![Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)](https://www.abcam.cn/ps/products/45/ab45152/Images/ab45152-12-benefits-of-recombinant-antibodies.png)
Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab45152 被引用在 11 文献中.
- Su L et al. Neuroprotective mechanism of TMP269, a selective class IIA histone deacetylase inhibitor, after cerebral ischemia/reperfusion injury. Neural Regen Res 15:277-284 (2020). PubMed: 31552900
- Jiang H et al. Opposing functions of Fng1 and the Rpd3 HDAC complex in H4 acetylation in Fusarium graminearum. PLoS Genet 16:e1009185 (2020). PubMed: 33137093
- Gómez-H L et al. The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility. PLoS Genet 15:e1008316 (2019). PubMed: 31437213
- Zeng Z et al. Poly (ADP-ribose) glycohydrolase silencing-mediated maintenance of H2A and downregulation of H2AK9me protect human bronchial epithelial cells from benzo(a)pyrene-induced carcinogenesis. Toxicol Lett 295:270-276 (2018). PubMed: 29981922
- Li H et al. A Phytophthora effector recruits a host cytoplasmic transacetylase into nuclear speckles to enhance plant susceptibility. Elife 7:N/A (2018). PubMed: 30346270