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Epigenetics and Nuclear Signaling Histones H2A Acetylated

Anti-Histone H2A (acetyl K5)抗体(ab1764)

  • Datasheet
Reviews (10)Q&A (4)References (12)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)

    Key features and details

    • Rabbit polyclonal to Histone H2A (acetyl K5)
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Histone H2A (acetyl K5)抗体
      参阅全部 Histone H2A 一抗
    • 描述

      兔多克隆抗体to Histone H2A (acetyl K5)
    • 宿主

      Rabbit
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Synthetic peptide corresponding to Histone H2A (acetyl K5) conjugated to ovalbumin.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.02% Sodium azide
    • Concentration information loading...
    • 纯度

      Whole antiserum
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • Histones
      • H2A
      • Acetylated

    相关产品

    • ChIP Related Products

      • Rabbit Anti-Mouse IgG H&L (ab46540)
      • ChIP Kit (ab500)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Histone H2A (biotinylated ) protein (ab200286)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1764于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (2)
    1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-P
    1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 功能

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 序列相似性

      Belongs to the histone H2A family.
    • 翻译后修饰

      The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
      Deiminated on Arg-4 in granulocytes upon calcium entry.
      Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
      Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
      Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    • 细胞定位

      Nucleus. Chromosome.
    • Target information above from: UniProt accession P04908 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 3012 Human
      • Entrez Gene: 317772 Human
      • Entrez Gene: 8335 Human
      • Entrez Gene: 8338 Human
      • Omim: 142720 Human
      • Omim: 602786 Human
      • Omim: 602797 Human
      • SwissProt: P04908 Human
      • SwissProt: P0C0S8 Human
      • SwissProt: P20671 Human
      • SwissProt: Q16777 Human
      • SwissProt: Q6FI13 Human
      • SwissProt: Q7L7L0 Human
      • SwissProt: Q8IUE6 Human
      • SwissProt: Q93077 Human
      • SwissProt: Q96KK5 Human
      • SwissProt: Q96QV6 Human
      • SwissProt: Q99878 Human
      • Unigene: 121017 Human
      • Unigene: 248174 Human
      see all
    • 别名

      • H2a 615 antibody
      • H2A antibody
      • H2A GL101 antibody
      • H2A histone family member A antibody
      • H2A.1 antibody
      • H2A.2 antibody
      • H2A/a antibody
      • H2A/m antibody
      • H2A/O antibody
      • H2A/q antibody
      • H2A1B_HUMAN antibody
      • H2AFA antibody
      • H2AFE antibody
      • H2AFL antibody
      • H2AFM antibody
      • H2AFO antibody
      • H2AFQ antibody
      • HIST1H2AE antibody
      • HIST1H2AJ antibody
      • HIST2H2AA antibody
      • HIST2H2AA3 antibody
      • HIST2H2AB antibody
      • HIST2H2AC antibody
      • Histone 1 H2ae antibody
      • Histone 2 H2aa3 antibody
      • Histone 2 H2ab antibody
      • Histone 2 H2ac antibody
      • Histone H2A type 1 B antibody
      • Histone H2A type 1 C antibody
      • Histone H2A type 1 E antibody
      • Histone H2A type 1 J antibody
      • Histone H2A type 1-B/E antibody
      • Histone H2A.2 antibody
      • Histone H2A/a antibody
      • Histone H2A/m antibody
      • MGC74460 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)

      IHC image of ab1764 staining Histone H2A (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1764, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (12)

    发表研究结果有使用 ab1764?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1764 被引用在 12 文献中.

    • Lau AC  et al. An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males. Epigenetics Chromatin 9:44 (2016). ICC/IF . PubMed: 27777629
    • Shirakawa K  et al. Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity. Elife 5:N/A (2016). WB . PubMed: 27244239
    • Min SW  et al. Critical role of acetylation in tau-mediated neurodegeneration and cognitive deficits. Nat Med 21:1154-62 (2015). PubMed: 26390242
    • Rothbart SB  et al. An Interactive Database for the Assessment of Histone Antibody Specificity. Mol Cell 59:502-11 (2015). PubMed: 26212453
    • Hu R  et al. Zinc finger protein 668 interacts with Tip60 to promote H2AX acetylation after DNA damage. Cell Cycle 12:2033-41 (2013). PubMed: 23777805
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 14 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2A (acetyl K5) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon cancer tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    Yes - Tween plus PBS
    Specification
    Colon cancer tissue
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 12 2021

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fruit fly embryo)
    Specification
    Fruit fly embryo
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 18 2013

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Mouse Cell lysate - nuclear (Mouse embryonic fibroblasts)
    Specification
    Mouse embryonic fibroblasts
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 18 2013

    IHC - Wholemount abreview for Anti-Histone H2A (acetyl K5) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Caenorhabditis elegans Tissue (Adult C. elegans whole body)
    Specification
    Adult C. elegans whole body
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 18 2013

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Caenorhabditis elegans Tissue lysate - whole (C. elegans embryo)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    20 µg
    Specification
    C. elegans embryo
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 18 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step
    Heat mediated
    Sample
    Mouse Tissue sections (Mouse brain)
    Specification
    Mouse brain
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 18 2013

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Keratinocytes)
    Loading amount
    50 µg
    Specification
    Keratinocytes
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 23 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (keratinocytes)
    Specification
    keratinocytes
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% triton-x-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 23 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Embryonic Stem)
    Specification
    Embryonic Stem
    Fixative
    Formaldehyde
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 20 2007

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (osteosarcoma (U2OS))
    Specification
    osteosarcoma (U2OS)
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 14 2006

    1-10 of 14 Abreviews or Q&A

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