重组Anti-Histone H1.3抗体[EPR12683] - BSA and Azide free (ab203948)
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)](https://www.abcam.cn/ps/products/203/ab203948/Images/ab203948-9-Anti-Histone-H1-3-antibody-EPR12683-BSA-and-Azide-free-immunocytochemistry-t47d-cells.png "Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12683] to Histone H1.3 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Histone H1.3抗体[EPR12683] - BSA and Azide free
参阅全部 Histone H1.3 一抗 -
描述
兔单克隆抗体[EPR12683] to Histone H1.3 - BSA and Azide free -
宿主
Rabbit -
特异性
The immunogen used for this product shares 6 continuous identical amino acids with H1.4. Cross-reactivity with this protein has not been confirmed experimentally.
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经测试应用
适用于: Flow Cyt (Intra), IHC-P, ICC/IF, IP, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: T47-D, HeLa & Jurkat whole cell lysate (ab7899), Human cervix carcinoma tissue, HeLa & T47-D cells Flow: Hela ICC: T47-D cells, Hela IHC: Human cervix carcinoma tissue IP: HeLa
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常规说明
ab203948 is the carrier-free version of ab183736.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12683 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab203948于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| IP |
1/30.
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| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
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| 说明 |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
Use at an assay dependent concentration. |
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IP
1/30. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
靶标
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功能
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
序列相似性
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 3007 Human
- GenBank: NP_005320 Human
- Omim: 142210 Human
- SwissProt: P16402 Human
- Unigene: 136857 Human
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别名
- H1 histone family member 3 antibody
- H1.3 antibody
- H13_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)This image is courtesy of an Abreview submitted by Albert JordanImmunocytochemistry/ Immunofluorescence analysis showing positive staining of H1.3 with ab203948 at 1/500 dilution in paraformaldehyde-fixed methanol permeabilized T47D cells, but not MDA-MB-231 breast cancer cells. The sample was incubated with the primary antibody for 16 hours at 4°C and blocking was done using 5% BSA for 1 hour at room temperature. Alexa Fluor® conjugated 488 goat anti-rabbit secondary antibody was used at 1/1000 dilution.
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![Western blot - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)](https://www.abcam.cn/ps/products/203/ab203948/Images/ab203948-410392-anti-histone-h13-antibody-epr12683-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)All lanes : Anti-Histone H1.3 antibody [EPR12683] (ab183736) at 1/5000 dilution
Lane 1 : T-47D cell lysate
Lane 2 : Hela cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDaThis data was developed using ab183736, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry (Intracellular) - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)](https://www.abcam.cn/ps/products/203/ab203948/Images/ab203948-4-anti-histone-h13-antibody-epr12683-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)This data was developed using ab183736, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of HeLa cells using ab183736 at 1/10 dilution. Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)](https://www.abcam.cn/ps/products/203/ab203948/Images/ab203948-3-anti-histone-h13-antibody-epr12683-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)This data was developed using ab183736, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of T47-D cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi -
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)](https://www.abcam.cn/ps/products/203/ab203948/Images/ab203948-2-anti-histone-h13-antibody-epr12683-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)](https://www.abcam.cn/ps/products/203/ab203948/Images/ab203948-1-anti-histone-h13-antibody-epr12683-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)This data was developed using ab183736, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Histone H1.3 with ab183736 at 1/1000 dilution. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Immunoprecipitation - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)](https://www.abcam.cn/ps/products/203/ab203948/Images/ab203948-491027-anti-histone-h13-antibody-EPR12683-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)Histone H1.3 was immunoprecipitated from 0.35 mg of HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate with ab183736 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183736 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: Hela whole cell lysate 10 μg (Input).
Lane 2: ab183736 IP in Hela whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183736 in Hela whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.
This data was developed using ab183736, the same antibody clone in a different buffer formulation.
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![Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)](https://www.abcam.cn/ps/products/203/ab203948/Images/ab203948-7-benefits-of-recombinant-antibodies.png)
Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (ab203948)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab203948 尚未被引用在任何文献中。
