重组Anti-Histone H1.3抗体[EPR12683] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (AB203948)

This data was developed using ab183736, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa cells using ab183736 at 1/10 dilution. Secondary ab : Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative : 2% paraformaldehyde. Isotype control : Rabbit monoclonal IgG.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (AB203948)

This data was developed using ab183736, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Histone H1.3 with ab183736 at 1/1000 dilution. Counter stain : Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (AB203948)

This data was developed using ab183736, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab : Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (AB203948)

This data was developed using ab183736, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of T47-D cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab : Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative : 4% paraformaldehyde. Counter stain : Dapi

• IHC-P

AbReview83609****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (AB203948)

Immunocytochemistry/ Immunofluorescence analysis showing positive staining of H1.3 with ab203948 at 1/500 dilution in paraformaldehyde-fixed methanol permeabilized T47D cells, but not MDA-MB-231 breast cancer cells. The sample was incubated with the primary antibody for 16 hours at 4 degrees and blocking was done using 5% BSA for 1 hour at room temperature. Alexa fluor® conjugated 488 goat anti-rabbit secondary antibody was used at 1/1000 dilution.

This image is courtesy of an Abreview submitted by Albert Jordan

• IP

Lab

Immunoprecipitation - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (AB203948)

This data was developed using ab183736, the same antibody clone in a different buffer formulation. Anti-Histone H1.3 was immunoprecipitated from 0.35 mg of HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate with ab183736 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183736 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Lane 1 : Hela whole cell lysate 10 μg (Input). Lane 2 : ab183736 IP in Hela whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab183736 in Hela whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 32 seconds.

All lanes:

Immunoprecipitation - Anti-Histone H1.3 antibody [EPR12683] (<a href='/products/primary-antibodies/histone-h13-antibody-epr12683-ab183736'>ab183736</a>)

Lane 1:

Hela whole cell lysate at 10 µg

Lane 2:

Anti-Histone H1.3 antibody IP in Hela whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 22 kDa

Observed band size: 30 kDa

false

Exposure time: 32s

• WB

Supplier Data

Western blot - Anti-Histone H1.3 antibody [EPR12683] - BSA and Azide free (AB203948)

This data was developed using ab183736, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Histone H1.3 antibody [EPR12683] (<a href='/products/primary-antibodies/histone-h13-antibody-epr12683-ab183736'>ab183736</a>) at 1/5000 dilution

Lane 1:

T-47D cell lysate at 20 µg

Lane 2:

Hela cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

false