重组Anti-Histone H1.1抗体[EPR23191-14] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.1 antibody [EPR23191-14] - BSA and Azide free (AB279642)

This data was developed using ab254394, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Histone H1.1 with ab254394 at 1/2000 (0.342 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human testis. The section was incubated with ab254394 overnight at 4°C. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.1 antibody [EPR23191-14] - BSA and Azide free (AB279642)

This data was developed using ab254394, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocelluar carcinoma tissue labelling Histone H1.1 with ab254394 at 1/2000 (0.342 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on human hepatocelluar carcinoma. The section was incubated with ab254394 overnight at 4°C. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.1 antibody [EPR23191-14] - BSA and Azide free (AB279642)

This data was developed using ab254394, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-468 cells labelling Histone H1.1 with ab254394 at 1/1000 (0.683 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MDA-MB-468 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Histone H1.1 antibody [EPR23191-14] - BSA and Azide free (AB279642)

This data was developed using ab254394, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-468 (Human breast adenocarcinoma epithelial cell) cells labelling Histone H1.1 with ab254394 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• WB

Lab

Western blot - Anti-Histone H1.1 antibody [EPR23191-14] - BSA and Azide free (AB279642)

This data was developed using 254394, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : MDA-MB-231.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-Histone H1.1 antibody [EPR23191-14] (<a href='/products/primary-antibodies/histone-h11-antibody-epr23191-14-ab254394'>ab254394</a>) at 1/1000 dilution

Lane 1:

MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

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• Dot

Supplier Data

Dot Blot - Anti-Histone H1.1 antibody [EPR23191-14] - BSA and Azide free (AB279642)

This data was developed using ab254394, the same antibody clone in a different buffer formulation.

Dot blot analysis of Histone H1.1 using ab254394 at 1/1000 (0.683 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate (ab97051) at 1/100,000 dilution.

Lane 1 : Histone H1.1 peptide

Lane 2 : Histone H1.2 peptide

Lane 3 : Histone H1.3 peptide

Lane 4 : Histone H1.4 peptide

Exposure time : 3 minutes.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.