Histone H1.0重组抗体[EPR6537]_Histone H1.0抗体(ab125027)

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Western blot - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6537] to Histone H1.0
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Human

Alexa Fluor® 488 Alexa Fluor® 594 Carrier Free

产品名称

Anti-Histone H1.0抗体[EPR6537]
参阅全部 Histone H1.0 一抗
描述

兔单克隆抗体[EPR6537] to Histone H1.0
宿主

Rabbit
经测试应用

适用于: WB, IHC-P, ICC/IFmore details
种属反应性

与反应: Human
预测可用于: Mouse, Rat
免疫原

Synthetic peptide within Human Histone H1.0 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P07305
阳性对照
- WB: A431 and BxPC3 cell lysates, and fetal human lung lysates IHC-P: Human kidney, pancreas, and thyroid tissues. ICC/IF: BxPC-3 and A431 cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR6537
同种型

IgG
研究领域

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human H1F0 knockout A-431 cell line (ab262478)
KO cell lysates
- Human H1F0 knockout A-431 cell lysate (ab263919)
Recombinant Protein
- Recombinant Human Histone H1.0 protein (ab158626)

The Abpromise guarantee

Abpromise™承诺保证使用ab125027于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 21 kDa).
IHC-P		1/100 - 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/250.

说明
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100 - 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

功能

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
序列相似性

Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
翻译后修饰

Phosphorylated on Ser-17 in RNA edited version.
细胞定位

Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
Target information above from: UniProt accession P07305 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 3005 Human
- Entrez Gene: 14958 Mouse
- Entrez Gene: 24437 Rat
- Omim: 142708 Human
- SwissProt: P07305 Human
- SwissProt: P10922 Mouse
- SwissProt: P43278 Rat
- Unigene: 745024 Human
- Unigene: 24350 Mouse
- Unigene: 3129 Rat
see all
别名
- H1 histone family member 0 antibody
- H1(0) antibody
- H10 antibody
- H10_HUMAN antibody
- h1f0 antibody
- H1FV antibody
- Histone H1'' antibody
- Histone H1(0) antibody
- Histone H1.0 antibody
- Histone H10 antibody
- Histone H5 antibody
- MGC5241 antibody
- N-terminally processed antibody
see all

Western blot - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

All lanes : Anti-Histone H1.0 antibody [EPR6537] (ab125027) at 1/1000 dilution

Lane 1 : Wild-type A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : H1F0 knockout A431 (Human epidermoid carcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 21 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?

Lanes 1 - 2: Merged signal (red and green). Green - ab125027 observed at 33 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab125027 was shown to react with H1F0 in A431 wild-type cells in Western blot. Loss of signal was observed when H1F0 knockout sample was used. A431 wild-type and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab125027 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

All lanes : Anti-Histone H1.0 antibody [EPR6537] (ab125027) at 10 µg (purified)

Lane 1 : human fetal lung lysate
Lane 2 : BxPC-3 lysate
Lane 3 : A431 lysate
Lane 4 : human fetal kidney lysate

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 21 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

Immunofluorescence staining of BxPC-3 cells with purified ab125027 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab125027 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

Immunohistochemical staining of paraffin embedded human thyroid carcinoma with purified ab125027 at a working dilution of 1/400. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

Immunohistochemical staining of paraffin embedded human pancreas with purified ab125027 at a working dilution of 1/400. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

All lanes : Anti-Histone H1.0 antibody [EPR6537] (ab125027) at 1/1000 dilution (unpurified)

Lane 1 : BxPC3 lysates
Lane 2 : Human fetal lung lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

Unpurified ab125027, at a 1/100 dilution, staining Histone H1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6537] (ab125027)

Unpurified ab125027, at a 1/100 dilution, staining Histone H1 in A431 cells by Immunofluorescence.
Anti-Histone H1.0 antibody [EPR6537] (ab125027)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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发表研究结果有使用 ab125027？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab125027 被引用在 14 文献中.

Jiang P et al. Apoptotic Neuron-Derived Histone Amyloid Fibrils Induce a-Synuclein Aggregation. Mol Neurobiol 58:867-876 (2021). PubMed: 33048264
Peng KY et al. Stromal Galectin-1 Promotes Colorectal Cancer Cancer-Initiating Cell Features and Disease Dissemination Through SOX9 and β-Catenin: Development of Niche-Based Biomarkers. Front Oncol 11:716055 (2021). PubMed: 34568045
Chatterjee A et al. MnTE-2-PyP, a manganese porphyrin, reduces cytotoxicity caused by irradiation in a diabetic environment through the induction of endogenous antioxidant defenses. Redox Biol 34:101542 (2020). PubMed: 32361681
Holokai L et al. Murine- and Human-Derived Autologous Organoid/Immune Cell Co-Cultures as Pre-Clinical Models of Pancreatic Ductal Adenocarcinoma. Cancers (Basel) 12:N/A (2020). PubMed: 33348809
Steele NG et al. An Organoid-Based Preclinical Model of Human Gastric Cancer. Cell Mol Gastroenterol Hepatol 7:161-184 (2019). PubMed: 30522949

View all Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Thank you for your response. Histone H1.2, 1.3 or 1.4 are about 21-22KD. Which of your antibody recognize these three isoforms, especially H1.4?
I also notice that you have Anti-Histone H1 (tri methyl K25) antibody (ab17347) which detects H1.4K25. But there is a post on your web site (question 2) states:"This antibody may not recognize methylated H1 but only the H3." Do you have any update for it? I am
interested to purchase this antibody if it indeed can recognize H1.4K25(K26).

Abcam community

Verified customer

Asked on Mar 06 2012

Answer

Thank you for contacting us.

H1.2 specific antibodies,
ab4086; https://www.abcam.com/Histone-H1-2-antibody-ChIP-Grade-ab4086.html. (we however haven't tested the specificity to other forms)
ab17677; https://www.abcam.com/Histone-H1-2-antibody-ab17677.html

H1.3 abs
ab24174; https://www.abcam.com/Histone-H1-3-antibody-ab24174.html
H1.4
ab105522; https://www.abcam.com/Histone-H1-4-antibody-ab105522.html
ab116393; https://www.abcam.com/Histone-H1-4-antibody-ab116393.html

The antibody ab17347 is for tri methylated H1, unfortunately it also cross react with methylated histone H3 and other isoform of H1. This antibody however will not recognize di and mono methylated histone H1. Is this the answer to your question? Let me know if you any further question about the specificity of these products.

Abcam Scientific Support

回复于 Mar 06 2012

Question

Would you please help to verify the following questions regarding your
anti-histone H1 anti bodies:
Anti-Histone H1 antibody (ab87556): Detects a ˜32KD band;
Anti-Histone H1 antibody (ab83058) : Detects a ˜24KD band;
Anti-Histone H1 antibody [1415-1] - Carboxyterminal end (ab62884) :
Detects a ˜17KD band;
Anti-Histone H1 antibody [AE-4] (ab71594) : no WB image;
Anti-Histone H1 antibody [EPR6537] (ab125027): WB ˜32KD
1). Do they represent different modifications of H1 or different isofroms
of H1, since the band sizes recognized are so diffenrent? (But MW of H1
isoforms are around 22KD).
2). Will above antibodies also cross-react with other histones like H3 or H4?
Anti-Histone H1 antibody [0.T.60] (ab4269): Detects isoforms 0-5,
˜25-32KD; Why the sizes are different from the oredicted sizes, or due to
modification or something else?
In the end, we need an antibody to detect Histone H1 in human cell lines
such as HEK293 and SH-SY5Y, which one do you recommend?
Thank you!

Abcam community

Verified customer

Asked on Mar 05 2012

Answer

Thank you for contacting us.

ab87556 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is 35 kDa.

ab83058; the sequence used is from P07305 http://www.uniprot.org/uniprot/P07305. The molecular weight is ˜ 21kDa.

ab71594 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is ˜ 35 kDa.

ab125027 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is ˜ 35 kDa.

These antibodies are specific to human H1 protein only not the modifications. You can purchase any of these to detect H1 human protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

回复于 Mar 05 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Isotype control

KO cell lines

KO cell lysates

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (14)

客户评价及客户问答