Anti-Histone H1.0抗体[34] (ab11079)
![Western blot - Anti-Histone H1.0 antibody [34] (ab11079)](https://www.abcam.cn/ps/products/11/ab11079/Images/ab11079-451728-anti-histone-h10-antibody-34-western-blot-wildtype-a431-triton-prep-mcf7-triton-prep-h1f0-knockout-a431-triton-pr.jpg "Western blot - Anti-Histone H1.0 antibody [34] (ab11079)")
Key features and details
- Mouse monoclonal [34] to Histone H1.0
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-Histone H1.0抗体[34]
参阅全部 Histone H1.0 一抗 -
描述
小鼠单克隆抗体[34] to Histone H1.0 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, Recombinant fragment
预测可用于: Mouse, Rat, Xenopus laevis, Vertebrata
不与反应:
Bird -
免疫原
Full length native protein (purified) corresponding to Cow Histone H1.0.
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表位
This antibody recognises an epitope within aa20-30. Proline 26 is not essential for recognition by the antibody. See Gorka et al. 1998 for more information. -
阳性对照
- WB: A431, MCF7 and HeLa cell lysate; Histone H1.0 (Human) - Recombinant Protein. IHC-P: Human breast adenocarcinama tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
34 -
骨髓瘤
NS1/1-Ag4-1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11079于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 20 kDa).
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 20 kDa). |
|
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. -
序列相似性
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
翻译后修饰
Phosphorylated on Ser-17 in RNA edited version. -
细胞定位
Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes. - Information by UniProt
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数据库链接
- Entrez Gene: 3005 Human
- Entrez Gene: 14958 Mouse
- Entrez Gene: 24437 Rat
- Omim: 142708 Human
- SwissProt: P07305 Human
- SwissProt: P10922 Mouse
- SwissProt: P43278 Rat
- Unigene: 745024 Human
see all -
别名
- H1 histone family member 0 antibody
- H1(0) antibody
- H10 antibody
see all
图片
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Western blot - Anti-Histone H1.0 antibody [34] (ab11079)All lanes : Anti-Histone H1.0 antibody [34] (ab11079) at 1/500 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : H1F0 knockout A431 cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab11079 observed at 30 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab11079 was shown to react with Histone H1.0 in wild-type A431 cells in Western blot with loss of signal observed in H1F0 knockout sample. Wild-type A431 and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab11079 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [34] (ab11079)](https://www.abcam.cn/ps/products/11/ab11079/Images/ab11079-293481-anti-histone-h10-antibody-34-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [34] (ab11079)IHC image of Histone H1.0 staining in a formalin fixed, paraffin embedded human breast adenocarcinama tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11079, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-Histone H1.0 antibody [34] (ab11079)](https://www.abcam.cn/ps/products/11/ab11079/Images/ab11079-293895-anti-histone-h10-antibody-34-western-blot.jpg)
Western blot - Anti-Histone H1.0 antibody [34] (ab11079)All lanes : Anti-Histone H1.0 antibody [34] (ab11079) at 1 µg/ml
Lane 1 : Histone H1.0 (Human) - Recombinant Protein
Lane 2 : Histone H1.2 (Human) - Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
![Western blot - Anti-Histone H1.0 antibody [34] (ab11079)](https://www.abcam.cn/ps/products/11/ab11079/Images/ab11079-293893-anti-histone-h10-antibody-34-western-blot.jpg)
Western blot - Anti-Histone H1.0 antibody [34] (ab11079)Anti-Histone H1.0 antibody [34] (ab11079) at 1 µg/ml + HeLa (Triton-enriched) Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
![Western blot - Anti-Histone H1.0 antibody [34] (ab11079)](https://www.abcam.cn/ps/products/11/ab11079/Images/ab11079_2.jpg)
Western blot - Anti-Histone H1.0 antibody [34] (ab11079)Western blot using ab11079 on HeLa whole cell extract (30
µ Exposure 5 sec using ECL.
12.5% SDS-PAGE gel. -
![Western blot - Anti-Histone H1.0 antibody [34] (ab11079)](https://www.abcam.cn/ps/products/11/ab11079/Images/ab11079_3.jpg)
Western blot - Anti-Histone H1.0 antibody [34] (ab11079)Dr Albert Jordan, Center for Genomic Regulation (CRG), Spain.Mouse monoclonal to Histone H1.0 against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure.
Dilution 1/1000.
Recombinant H1 isoforms courtesy of Dr Nicole Happel.
(see attached review)
数据表及文件
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SDS download
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Datasheet download
文献 (34)
ab11079 被引用在 34 文献中.
- Gómora-García JC et al. Effect of the Ketone Body, D-β-Hydroxybutyrate, on Sirtuin2-Mediated Regulation of Mitochondrial Quality Control and the Autophagy-Lysosomal Pathway. Cells 12:N/A (2023). PubMed: 36766827
- Bhandari SK et al. Unchanged PCNA and DNMT1 dynamics during replication in DNA ligase I-deficient cells but abnormal chromatin levels of non-replicative histone H1. Sci Rep 13:4363 (2023). PubMed: 36928068
- Ozgencil M et al. The linker histone H1-BRCA1 axis is a crucial mediator of replication fork stability. Life Sci Alliance 6:N/A (2023). PubMed: 37364916
- Serna-Pujol N et al. Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion. Nucleic Acids Res 50:3892-3910 (2022). PubMed: 35380694
- Yang Z et al. Forsythiaside Protected H9c2 Cardiomyocytes from H2O2-Induced Oxidative Stress and Apoptosis via Activating Nrf2/HO-1 Signaling Pathway. Int Heart J 63:904-914 (2022). PubMed: 36184550