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Epigenetics and Nuclear Signaling Histones H1 Phosphorylated

Anti-Histone H1 (phospho )抗体(ab4270)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (7)

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Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (phospho ) antibody (ab4270)
  • Western blot - Anti-Histone H1 (phospho ) antibody (ab4270)

Key features and details

  • Rabbit polyclonal to Histone H1 (phospho )
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Histone H1 (phospho )抗体
  • 描述

    兔多克隆抗体to Histone H1 (phospho )
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, Flow Cyt, WBmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Hyper-phosphorylated isoform of tetrahymena Histone H1

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.035% Sodium azide
    Constituents: 1.3% Tris glycine, 30% Glycerol, 0.667% Sodium chloride
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Histones
    • H1
    • Phosphorylated

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Histone H1 protein (ab198676)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4270于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100 - 1/100.
Flow Cyt
Use at an assay dependent concentration. PubMed: 18250156

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa. Additional proteins at 66 kD and 100 kD were detected. It detected Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- Histone H1 (2 µg/ml).
说明
ICC/IF
1/100 - 1/100.
Flow Cyt
Use at an assay dependent concentration. PubMed: 18250156

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa. Additional proteins at 66 kD and 100 kD were detected. It detected Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- Histone H1 (2 µg/ml).

靶标

  • 功能

    Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • 序列相似性

    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • 翻译后修饰

    Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
  • 细胞定位

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P10412 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3008 Human
    • Entrez Gene: 50709 Mouse
    • Omim: 142711 Human
    • SwissProt: P10412 Human
    • SwissProt: P16401 Human
    • SwissProt: P43274 Mouse
    • SwissProt: P43276 Mouse
    • Unigene: 248133 Human
    • Unigene: 170587 Mouse
    see all
  • 别名

    • H1 histone family antibody
    • H14_HUMAN antibody
    • H1F5 antibody
    • Hist1h1e antibody
    • Histone H1.4 antibody
    • Histone H1a antibody
    • Histone H1B antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (phospho ) antibody (ab4270)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (phospho ) antibody (ab4270)This image is courtesy of Dr Michael Hendzels Laboratory

    Rabbit polyclonal to Histone H1 (phospho) (1/100).

    Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab4270 (green). The DNA stained with DAPI is shown in red. (100x magnification).

  • Western blot - Anti-Histone H1 (phospho ) antibody (ab4270)
    Western blot - Anti-Histone H1 (phospho ) antibody (ab4270)
    All lanes : Anti-Histone H1 (phospho ) antibody (ab4270) at 2 µg/ml

    Lane 1 : Colcemid treated HeLa cell lysate
    Lane 2 : Untreated HeLa cell lysate

    Secondary
    Lane 1 : Goat anti-rabbit
    secondary antibody labeled with HRP
    Lane 2 : Goat anti-rabbit
    secondary antibody labeled with HRP.

    Developed using the ECL technique.

实验方案

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (7)

发表研究结果有使用 ab4270?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab4270 被引用在 7 文献中.

  • Murthy T  et al. Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin. J Biol Chem 293:10220-10234 (2018). PubMed: 29764937
  • Zhang B  et al. Silybin attenuates LPS-induced lung injury in mice by inhibiting NF-?B signaling and NLRP3 activation. Int J Mol Med 39:1111-1118 (2017). PubMed: 28350048
  • Lei X  et al. Neuroprotective effects of lycopene pretreatment on transient global cerebral ischemia-reperfusion in rats: The role of the Nrf2/HO-1 signaling pathway. Mol Med Rep 13:412-8 (2016). PubMed: 26572165
  • Büchner A  et al. Sumoylation of p35 modulates p35/cyclin-dependent kinase (Cdk) 5 complex activity. Neuromolecular Med 17:12-23 (2015). PubMed: 25391294
  • Chien WW  et al. S-phase lengthening induced by p16(INK4a) overexpression in malignant cells with wild-type pRb and p53. Cell Cycle 9: (2010). WB ; Human . PubMed: 20703084
  • Ingemarsdotter CK  et al. Low-dose paclitaxel synergizes with oncolytic adenoviruses via mitotic slippage and apoptosis in ovarian cancer. Oncogene 29:6051-63 (2010). WB ; Human . PubMed: 20729921
  • Tang X  et al. A novel ATM-dependent pathway regulates protein phosphatase 1 in response to DNA damage. Mol Cell Biol 28:2559-66 (2008). Flow Cyt ; Human . PubMed: 18250156

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Histone H1 (phospho) antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Parotid gland)
Loading amount
100 µg
Specification
Parotid gland
Treatment
5Gy gamma irradiation
Gel Running Conditions
Non-reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1500µg/mL · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Kirsten Limesand

Verified customer

提交于 Nov 16 2010

Question

I am wondering if you can tell me more about this antibody:
Anti-Histone H1 (phospho) antibody (ab4270)
Specifically, what phosphorylated residue does the antibody recognize?

Read More

Abcam community

Verified customer

Asked on Feb 24 2012

Answer



Concerningab4270, Histone H1, phosphorylated PabRabbit x:



Thisantibody is produced by using hyper-phosphorylated histone H1 as the immunogen. The number and location of phosphorylation sites that thisantibody recognizes have not been determined. Thisantibody has been used to monitor passage through mitosis and the signal is maximal at metaphase. The presence ofhyper-phosphorylated histone H1has also been used as a measure of cdck1 activity.



There is no single specific phosphorylation site on H1 that is required for recognition by ab4270.

Read More

Abcam Scientific Support

回复于 Feb 24 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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