重组Anti-HIRA/HIR抗体[EPR25299-11] (ab302928)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25299-11] to HIRA/HIR
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-HIRA/HIR抗体[EPR25299-11]
参阅全部 HIRA/HIR 一抗 -
描述
兔单克隆抗体[EPR25299-11] to HIRA/HIR -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details
不适用于: ChIP,Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: F9 transfected with unscrambled and scrambled (control) siRNA specifically targeting HIRA, whole cell lysates; human testis tissue lysates; mouse and rat brain and cerebellum tissue lysates; mouse lung tissue lysate. IHC-P: Mouse and rat cerebrum, mouse lung and human skin tissue. IP: F9 and HeLa whole cell lysate.
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常规说明
AB302928 does not react with human or mouse samples in ICC/IF or Flow Cytometry (Intra). It does not react with mouse samples in ChIP.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR25299-11 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab302928于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 112 kDa).
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IP |
1/30.
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说明 |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 112 kDa). |
IP
1/30. |
靶标
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功能
Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. -
组织特异性
Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta. -
序列相似性
Belongs to the WD repeat HIR1 family.
Contains 8 WD repeats. -
发展阶段
Expressed during embryogenesis. -
翻译后修饰
Sumoylated.
Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo. -
细胞定位
Nucleus. Nucleus > PML body. Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence. - Information by UniProt
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数据库链接
- Entrez Gene: 7290 Human
- Entrez Gene: 15260 Mouse
- Entrez Gene: 363849 Rat
- Omim: 600237 Human
- SwissProt: P54198 Human
- SwissProt: Q61666 Mouse
- Unigene: 474206 Human
- Unigene: 15694 Mouse
see all -
别名
- DGCR1 antibody
- DiGeorge critical region gene 1 antibody
- HIR antibody
see all
图片
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All lanes : Anti-HIRA/HIR antibody [EPR25299-11] (ab302928) at 1/1000 dilution
Lane 1 : F9 (mouse embryonal carcinoma epithelial cell), transfected with scrambled siRNA control, whole cell lysate
Lane 2 : F9 transfected with siRNA specifically targeting HIRA, whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution
Predicted band size: 112 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking / Diluting buffer: 5% NFDM/TBST.
The bands beneath the target band (110KD) are likely to be degradation.
Exposure time: 7.75 seconds.
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All lanes : Anti-HIRA/HIR antibody [EPR25299-11] (ab302928) at 1/1000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Human lung tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Rat brain tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 112 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking / Diluting buffer: 5% NFDM/TBST.
Exposure times: Lane 1-3: 114 seconds; Lane 4 and 5: 37 seconds.
Low expression: human liver, human lung (PMID: 9063745).
The bands beneath the target band (110KD) are likely to be degradation. -
All lanes : Anti-HIRA/HIR antibody [EPR25299-11] (ab302928) at 1/1000 dilution
Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Rat cerebellum tissue lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 112 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking / Diluting buffer: 5% NFDM/TBST.
The bands beneath the target band (110KD) are likely to be degradation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling HIRA/HIR with ab302928 at 1/100 dilution (4.86 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Nuclear staining on mouse cerebrum. The section was incubated with ab302928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: A ready to use Leica DS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling HIRA/HIR with ab302928 at 1/100 dilution (4.86 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Nuclear staining on mouse lung. The section was incubated with ab302928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: A ready to use Leica DS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling HIRA/HIR with ab302928 at 1/100 dilution (4.86 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Nuclear staining on rat cerebrum. The section was incubated with ab302928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: A ready to use Leica DS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIRA/HIR antibody [EPR25299-11] (AB302928)
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling HIRA/HIR with ab302928 at 1/100 dilution (4.86 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Nuclear staining on human skin. The section was incubated with ab302928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: A ready to use Leica DS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.
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HIRA/HIR was immunoprecipitated from 0.35 mg F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate 10 µg with ab302928 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab302928 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: F9 whole cell lysate 10 µg
Lane 2: ab302928 IP in F9 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302928 in F9 whole cell lysate
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 180 seconds.
The bands beneath the target band (110KD) are likely to be degradation.
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HIRA/HIR was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab302928 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab302928 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 µg
Lane 2: ab302928 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302928 in HeLa whole cell lysate
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 180 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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