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重组RabMAb

重组Anti-HIPK2抗体[EPR3819] (ab108543)

Submit a review Q&A (1)References (8)
Western blot - Anti-HIPK2 antibody [EPR3819] (ab108543)
  • Flow Cytometry (Intracellular) - Anti-HIPK2 antibody [EPR3819] (ab108543)
  • Western blot - Anti-HIPK2 antibody [EPR3819] (ab108543)
  • Anti-HIPK2 antibody [EPR3819] (ab108543)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3819] to HIPK2
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

概述

  • 产品名称

    Anti-HIPK2抗体[EPR3819]
    参阅全部 HIPK2 一抗

  • 描述

    兔单克隆抗体[EPR3819] to HIPK2

  • 宿主

    Rabbit

  • 特异性

    The immunogen used for this product shares 69% homology and 6 consecutive amino acids with HIPK1. Cross-reactivity with this protein has not been confirmed experimentally.

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HT-1080 and HepG2 whole cell lysate (ab7900)

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab108543于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/2000 - 1/10000. Detects a band of approximately 100, 130 kDa (predicted molecular weight: 131 kDa).
说明
Flow Cyt (Intra)
1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/2000 - 1/10000. Detects a band of approximately 100, 130 kDa (predicted molecular weight: 131 kDa).
应用说明
Is unsuitable for IHC-P.

靶标

  • 功能

    Protein kinase acting as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. Phosphorylation of TP53 may be mediated by a TP53-HIPK2-AXIN1 complex. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB (By similarity). Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.

  • 组织特异性

    Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.

  • 序列相似性

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.

  • 翻译后修饰

    Phosphorylated on tyrosines (By similarity). Autophosphorylated.
    Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.
    Ubiquitinated by FBXO3, leading to rapid proteasome-dependent degradation. This degradation, but not ubiquitination, is prevented in the presence of PML.

  • 细胞定位

    Nucleus > PML body. Cytoplasm. Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.
  • Information by UniProt

  • 数据库链接

    • 别名

      • hHIPk 2 antibody
      • hHIPk2 antibody
      • HIPK 2 antibody
      • Hipk2 antibody
      • HIPK2_HUMAN antibody
      • Homeodomain interacting protein kinase 2 antibody
      • Homeodomain-interacting protein kinase 2 antibody
      • Nbak1 antibody
      • Nuclear body-associated kinase 1 antibody
      • PRO0593 antibody
      • Sialophorin tail-associated nuclear serine/threonine-protein kinase antibody
      • Stank antibody
      see all

    图片

    • Western blot - Anti-HIPK2 antibody [EPR3819] (ab108543)
      Western blot - Anti-HIPK2 antibody [EPR3819] (ab108543)
      Anti-HIPK2 antibody [EPR3819] (ab108543) at 1/2000 dilution + Mouse heart tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Predicted band size: 131 kDa
      Observed band size: 100,130 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    • Flow Cytometry (Intracellular) - Anti-HIPK2 antibody [EPR3819] (ab108543)
      Flow Cytometry (Intracellular) - Anti-HIPK2 antibody [EPR3819] (ab108543)

      Overlay histogram showing HEK293 cells stained with ab108543 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108543, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Western blot - Anti-HIPK2 antibody [EPR3819] (ab108543)
      Western blot - Anti-HIPK2 antibody [EPR3819] (ab108543)
      All lanes : Anti-HIPK2 antibody [EPR3819] (ab108543) at 1/2000 dilution

      Lane 1 : HT-1080 cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 131 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa (possible isoform)

    • Anti-HIPK2 antibody [EPR3819] (ab108543)
      Anti-HIPK2 antibody [EPR3819] (ab108543)

    文献 (8)

    发表研究结果有使用 ab108543？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab108543 被引用在 8 文献中.

    • Zhang Q  et al. HMGB1-induced activation of ER stress contributes to pulmonary artery hypertension in vitro and in vivo. Respir Res 24:149 (2023). PubMed: 37268944
    • Huang H  et al. Adipose-derived stem cell exosome NFIC improves diabetic foot ulcers by regulating miR-204-3p/HIPK2. J Orthop Surg Res 18:687 (2023). PubMed: 37710299
    • Li X  et al. SIRT6 overexpression retards renal interstitial fibrosis through targeting HIPK2 in chronic kidney disease. Front Pharmacol 13:1007168 (2022). PubMed: 36172184
    • Zhou Q  et al. Exercise downregulates HIPK2 and HIPK2 inhibition protects against myocardial infarction. EBioMedicine 74:103713 (2021). PubMed: 34837851
    • Li Y  et al. SPEN induces miR-4652-3p to target HIPK2 in nasopharyngeal carcinoma. Cell Death Dis 11:509 (2020). PubMed: 32641685
    • Fisher JN  et al. MicroRNA networks regulated by all-trans retinoic acid and Lapatinib control the growth, survival and motility of breast cancer cells. Oncotarget 6:13176-200 (2015). WB . PubMed: 25961594
    • Cozza G  et al. Synthesis and properties of a selective inhibitor of homeodomain-interacting protein kinase 2 (HIPK2). PLoS One 9:e89176 (2014). IP ; Human . PubMed: 24586573
    • Dacheux E  et al. BRCA1-Dependent Translational Regulation in Breast Cancer Cells. PLoS One 8:e67313 (2013). WB ; Human . PubMed: 23805307

    客户评价及客户问答

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    Question

    Why there is no band in HepG2 lysates?

    Read More
    Answer

    There are 3 isoforms of this protein with mwt 131kDa, 100kDa and 128kDa. The antibody is detecting the 100kDa isoform in hepG2 lysates.


    I am also CC'ing our Custom Services group on this message and they will set up a call with you to discuss your experimental needs further. They will collaborate with you from this point and they may ask some additional questions like your target and application of interest.

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