重组Anti-HIPK2抗体[EPR3819]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HIPK2 antibody [EPR3819] (AB108543)

Overlay histogram showing HEK293 cells stained with ab108543 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108543 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• WB

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Western blot - Anti-HIPK2 antibody [EPR3819] (AB108543)

All lanes:

Western blot - Anti-HIPK2 antibody [EPR3819] (ab108543) at 1/2000 dilution

Lane 1:

HT-1080 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 131 kDa

Observed band size: 100 kDa,130 kDa

false

• WB

Lab

Western blot - Anti-HIPK2 antibody [EPR3819] (AB108543)

Blocking and diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-HIPK2 antibody [EPR3819] (ab108543) at 1/2000 dilution

All lanes:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 131 kDa

Observed band size: 100 kDa,130 kDa

false

Exposure time: 180s

• WB

CiteAb

Western blot - Anti-HIPK2 antibody [EPR3819] (AB108543)

HIPK2 western blot using anti-HIPK2 antibody [EPR3819] ab108543. Publication image and figure legend from Li, Y., Lv, Y., et al., 2020, Cell Death Dis, PubMed 32641685.

ab108543 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab108543 please see the product overview.

miR-4652-3p directly targets HIPK2.a Targetscan, miRWalk, and mirDIP were used to predict target genes of miR-4652-3p. b HIPK2 expression was examined in miR-4652-3p-overexpressing or miR-4652-3p-inhibited cells by western blot, β-actin was used as a loading control. c Luciferase reporter assay was used to determine miR-4652-3p directly targets the 3'UTR of HIPK2. *p < 0.05. d Changes of E-cadherin, Vimentin and HIPK2 expression were detected by western blot in HONE1 and 5–8F cell lines after transfection of miR-4652-3p inhibitor or HIPK2 siRNAs. e Western blot of HIPK2 protein expression in HONE1 and 5–8F cells treated with SPEN siRNAs or corresponding control. β-actin served as a loading control.

false