AB264376

Anti-HIP1抗体

Name: HIP1抗体 (ab264376)| Abcam中文官网
Brand: Abcam Limited
SKU: AB264376
Price: 4863 CNY
Availability: InStock

Anti-HIP1 antibody

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Rabbit Polyclonal HIP1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HIP1 aa 700-800.

查看别名

Huntingtin-interacting protein 1, HIP-1, Huntingtin-interacting protein I, HIP-I, HIP1

2 Images

Supplier Data

Immunoprecipitation - Anti-HIP1 antibody (AB264376)

HIP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using ab264376 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264376 at 1 μg/ml.

Lane 1 : ab264376 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in Hela whole cell lysate.

Exposure time : 30 seconds.

Lysates prepared in NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-HIP1 antibody (ab264376)

Predicted band size: 116 kDa

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Supplier Data

Western blot - Anti-HIP1 antibody (AB264376)

Lysates prepared in NETN lysis buffer.

All lanes:

Western blot - Anti-HIP1 antibody (ab264376) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 116 kDa

true

Exposure time: 10s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human HIP1 aa 700-800. The exact immunogen used to generate this antibody is proprietary information.

O00291

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HIP1 also known as Huntingtin-interacting protein 1 is a protein with a mass of approximately 116 kDa. HIP1 is an adapter protein involved in the endocytosis process. It interacts with clathrin and AP2 adaptor complex assisting in the invagination and fission of clathrin-coated pits. HIP1 is present throughout various tissues including the brain and immune cells indicating its widespread role in cellular mechanisms.

Biological function summary

HIP1 plays an essential role in the modulation of receptor-mediated endocytosis. It is part of a larger complex with epsin and other proteins facilitating the internalization of extracellular signals. The protein's interaction with actin and lipid membranes suggests its function in reorganizing cytoskeleton arrangements vital for cell movement and shape changes. Cellular homeostasis and signal transduction rely on HIP1's ability to mediate these activities.

Pathways

HIP1 integrates into the PI3K/AKT signaling impacting cell survival and apoptosis. Its activity in clathrin-mediated endocytosis also links it to the receptor tyrosine kinase (RTK) pathway. In these pathways HIP1 assists in the regulation of growth factor receptors collaborating with proteins such as GRB2 and SHC1. These interactions ensure proper cellular responses to external stimuli.

HIP1 has associations with cancer and neurodegenerative diseases. In cancer overexpression of HIP1 enhances cell proliferation linked to its role in receptor trafficking and signaling. HIP1's interaction with huntingtin the protein mutated in Huntington's disease suggests its involvement in neurodegeneration. Such connections emphasize the protein's potential as a target for therapeutic intervention highlighting HIP1's impact on pathological conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in clathrin-mediated endocytosis and trafficking (PubMed : 11532990, PubMed : 11577110, PubMed : 11889126). Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner (By similarity). Regulates presynaptic nerve terminal activity (By similarity). Enhances androgen receptor (AR)-mediated transcription (PubMed : 16027218). May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway (PubMed : 11007801). Binds 3-phosphoinositides (via ENTH domain) (PubMed : 14732715). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis (PubMed : 14732715). May play a functional role in the cell filament networks (PubMed : 18790740). May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors (PubMed : 11007801, PubMed : 12163454).

See full target information HIP1

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Anti-HIP1抗体

Anti-HIP1 antibody

Immunoprecipitation - Anti-HIP1 antibody (AB264376)

Western blot - Anti-HIP1 antibody (AB264376)