Anti-HINT3抗体(ab121960)
Key features and details
- Rabbit polyclonal to HINT3
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HINT3抗体 -
描述
兔多克隆抗体to HINT3 -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: MAEEQVNRSA GLAPDCEASA TAETTVSSVG TCEAAAKSPE PKDYDSTCVF CRIAGRQDPG TELLHCENED LICFKDIKPA ATHHYLVVP, corresponding to amino acids 1-89 of Human HINT3 (UniProt Q9NQE9).
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阳性对照
- WB: RT4 cell lysate. ICC: A431 cells. IHC-P: Human prostate, cerebral cortex and colon tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121960于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 0.25 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval via the pressure cooker method.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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ICC
Use a concentration of 0.25 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval via the pressure cooker method. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
Hydrolyzes phosphoramidate and acyl-adenylate substrates. -
序列相似性
Belongs to the HINT family.
Contains 1 HIT domain. -
细胞定位
Cytoplasm. Nucleus. Localized as aggregates in the cytoplasm and the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 135114 Human
- Omim: 609998 Human
- SwissProt: Q9NQE9 Human
- Unigene: 72325 Human
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别名
- HINT-3 antibody
- HINT3 antibody
- HINT3_HUMAN antibody
- Histidine triad nucleotide-binding protein 3 antibody
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT3 antibody (ab121960)
Immunohistochemical analysis of human colon tissue labeling HINT3 with ab121960 at a 1/200 dilution.
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Immunocytochemistry - Anti-HINT3 antibody (ab121960)Immunocytochemistry of A431 (Human epidermoid carcinoma) cells labeling HINT3 with ab121960 at 0.25 ug/ml.
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Western blot - Anti-HINT3 antibody (ab121960)Anti-HINT3 antibody (ab121960) at 0.04 µg/ml + RT4 (Human urinary bladder cancer cell line) whole cell lysateWestern blot analysis of RT-4 cells labeling HINT3 with ab121960 at 0.04 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT3 antibody (ab121960)Immunohistochemical analysis of human prostate tissue labeling HINT3 with ab121960 at a 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT3 antibody (ab121960)Immunohistochemical analysis of human cerebral cortex tissue labeling HINT3 with ab121960 at a 1/200 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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