重组Anti-HINT1抗体[EPR5108] (ab124912)
![Western blot - Anti-HINT1 antibody [EPR5108] (ab124912)](https://www.abcam.cn/ps/products/124/ab124912/Images/ab124912-393615-anti-hint1-antibody-epr5108-western-blot-human-knockout-lysate.jpg "Western blot - Anti-HINT1 antibody [EPR5108] (ab124912)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5108] to HINT1
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HINT1抗体[EPR5108]
参阅全部 HINT1 一抗 -
描述
兔单克隆抗体[EPR5108] to HINT1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human HINT1. The exact sequence is proprietary.
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阳性对照
- WB: HeLa, 293T, MCF7, HepG2 and Jurkat cell lysates. IHC-P: Human breast carcinoma and Human colonic adenocarcinoma tissues. ICC/IF: MCF7 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5108 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124912于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
|
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Heat to 98°C, allow to cool for 10-20 minutes) |
|
| ICC/IF |
1/1000.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
|
ICC/IF
1/1000. |
靶标
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功能
Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the HINT family.
Contains 1 HIT domain. -
结构域
The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide. -
细胞定位
Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization. - Information by UniProt
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数据库链接
- Entrez Gene: 3094 Human
- Entrez Gene: 15254 Mouse
- Entrez Gene: 690660 Rat
- Omim: 601314 Human
- SwissProt: P49773 Human
- SwissProt: P70349 Mouse
- SwissProt: P62959 Rat
- Unigene: 483305 Human
see all -
别名
- Adenosine 5' monophosphoramidase antibody
- Adenosine 5''-monophosphoramidase antibody
- HINT 1 antibody
see all
图片
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Western blot - Anti-HINT1 antibody [EPR5108] (ab124912)All lanes : Anti-HINT1 antibody [EPR5108] (ab124912) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : HINT1 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab124912 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa.
ab124912 Anti-HINT1 antibody [EPR5108] was shown to specifically react with HINT1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265776 (knockout cell lysate ab257465) was used. Wild-type and HINT1 knockout samples were subjected to SDS-PAGE. ab124912 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-HINT1 antibody [EPR5108] (ab124912)](https://www.abcam.cn/ps/products/124/ab124912/Images/ab124912-138905-anti-hint1-antibody-epr5108-western-blot.jpg)
Western blot - Anti-HINT1 antibody [EPR5108] (ab124912)All lanes : Anti-HINT1 antibody [EPR5108] (ab124912) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 14 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-HINT1 antibody [EPR5108] (ab124912)](https://www.abcam.cn/ps/products/124/ab124912/Images/ab124912-4-anti-hint1-antibody-epr5108-immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HINT1 antibody [EPR5108] (ab124912)Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling HINT1 with purified ab124912 at 1/1000 dilution (2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] (ab124912)](https://www.abcam.cn/ps/products/124/ab124912/Images/ab124912-138904-anti-hint1-antibody-epr5108-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] (ab124912)ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded breast carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] (ab124912)](https://www.abcam.cn/ps/products/124/ab124912/Images/ab124912-138903-anti-hint1-antibody-epr5108-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] (ab124912)ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded colonic adenocarcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-HINT1 antibody [EPR5108] (ab124912)](https://www.abcam.cn/ps/products/124/ab124912/Images/ab124912-6-benefits-of-recombinant-antibodies.png)
Anti-HINT1 antibody [EPR5108] (ab124912)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab124912 被引用在 7 文献中.
- Zhang C et al. Integrated analysis of ceRNA network reveals potential prognostic Hint1-related lncRNAs involved in hepatocellular carcinoma progression. World J Surg Oncol 20:67 (2022). PubMed: 35241097
- Liu F et al. HINT1 Is Involved in the Chronic Mild Stress Elicited Oxidative Stress and Apoptosis Through the PKC e/ALDH-2/4HNE Pathway in Prefrontal Cortex of Rats. Front Behav Neurosci 15:690344 (2021). PubMed: 34177485
- Schwenzer H et al. The Novel Nucleoside Analogue ProTide NUC-7738 Overcomes Cancer Resistance Mechanisms In Vitro and in a First-In-Human Phase I Clinical Trial. Clin Cancer Res 27:6500-6513 (2021). PubMed: 34497073
- Sato M et al. The lncRNA Caren antagonizes heart failure by inactivating DNA damage response and activating mitochondrial biogenesis. Nat Commun 12:2529 (2021). PubMed: 33953175
- Jiang K et al. Genetic Fine Mapping and Genomic Annotation Defines Causal Mechanisms at A Novel Colorectal Cancer Susceptibility Locus in Han Chinese. J Cancer 11:6841-6849 (2020). PubMed: 33123275
- Yu J et al. Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FceRI-activated mast cells. Nat Commun 10:4664 (2019). PubMed: 31604935
- Zhou Y et al. Altered Light Conditions Contribute to Abnormalities in Emotion and Cognition Through HINT1 Dysfunction in C57BL/6 Mice. Front Behav Neurosci 12:110 (2018). PubMed: 29937721