Anti-HIF-2-alpha抗体[ep190b] (ab8365)
![Western blot - Anti-HIF-2-alpha antibody [ep190b] (ab8365)](https://www.abcam.cn/ps/products/8/ab8365/Images/ab8365-341639-ID158Ab8365Ap3897375annotated.jpg "Western blot - Anti-HIF-2-alpha antibody [ep190b] (ab8365)")
Key features and details
- Mouse monoclonal [ep190b] to HIF-2-alpha
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-HIF-2-alpha抗体[ep190b]
参阅全部 HIF-2-alpha 一抗 -
描述
小鼠单克隆抗体[ep190b] to HIF-2-alpha -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Hypoxic A549 and HeLa cell lysate.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
ep190b -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa.
Abcam recommends using a 3% milk block with this product. |
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa. Abcam recommends using a 3% milk block with this product. |
靶标
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功能
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. -
组织特异性
Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells. -
疾病相关
Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
翻译后修饰
In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
Phosphorylated on multiple sites in the CTAD.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2034 Human
- Entrez Gene: 29452 Rat
- Omim: 603349 Human
- SwissProt: Q99814 Human
- SwissProt: Q9JHS1 Rat
- Unigene: 468410 Human
- Unigene: 55138 Rat
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别名
- Basic helix loop helix PAS protein MOP2 antibody
- Basic-helix-loop-helix-PAS protein MOP2 antibody
- bHLHe73 antibody
see all
图片
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Western blot - Anti-HIF-2-alpha antibody [ep190b] (ab8365)All lanes : Anti-HIF-2-alpha antibody [ep190b] (ab8365) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa treated with 0.5mM DFO whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab8365 overnight at 4°C. Antibody binding was detected using a Goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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![Western blot - Anti-HIF-2-alpha antibody [ep190b] (ab8365)](https://www.abcam.cn/ps/products/8/ab8365/Images/ab8365-14153-anti-hif-2-alpha-antibody-ep190b-western-blot.jpg)
Western blot - Anti-HIF-2-alpha antibody [ep190b] (ab8365)HIF-2-alpha detected in hypoxic Human lysate using ab8365. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (71)
ab8365 被引用在 71 文献中.
- Chee NT et al. Activating transcription factor 4 regulates hypoxia inducible factor 1α in chronic hypoxia in pancreatic cancer cells. Oncol Rep 49:N/A (2023). PubMed: 36416348
- Goncalves J et al. Loss of SDHB Promotes Dysregulated Iron Homeostasis, Oxidative Stress, and Sensitivity to Ascorbate. Cancer Res 81:3480-3494 (2021). PubMed: 34127497
- Arroyo N et al. GATA4 induces liver fibrosis regression by deactivating hepatic stellate cells. JCI Insight 6:N/A (2021). PubMed: 34699385
- Shih HJ et al. Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer. Sci Rep 11:22925 (2021). PubMed: 34824343
- Morin A et al. TET-Mediated Hypermethylation Primes SDH-Deficient Cells for HIF2a-Driven Mesenchymal Transition. Cell Rep 30:4551-4566.e7 (2020). PubMed: 32234487