AB243861

Anti-HIF-2-alpha 抗体 [BL-95-1A2]

Name: HIF-2-alpha抗体[BL-95-1A2] (ab243861)| Abcam中文官网
Brand: Abcam Limited
SKU: AB243861
Price: 5261 CNY
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Rating: 1 (1 reviews)

Anti-HIF-2-alpha antibody [BL-95-1A2]

Advanced Validation
Recombinant
了解详情

(1 Review)

(15 Publications)

Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861) is a rabbit monoclonal antibody detecting HIF-2-alpha in Western Blot, IP, IHC-P, ICC/IF, ChIP-seq. Suitable for Human.

- Recombinant format for unrivalled batch-batch consistency

查看别名

BHLHE73, HIF2A, MOP2, PASD2, EPAS1, Endothelial PAS domain-containing protein 1, EPAS-1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, HIF-1-alpha-like factor, Hypoxia-inducible factor 2-alpha, Member of PAS protein 2, PAS domain-containing protein 2, bHLHe73, HLF, HIF-2-alpha, HIF2-alpha

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody [BL-95-1A2] (AB243861)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody [BL-95-1A2] (AB243861)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for HIF-2 alpha using ab243861 at 1/400 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

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Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] (AB243861)

Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling HIF-2 alpha using ab243861 at 1/400 dilution in ICC/IF analysis. A HRP-conjugated goat-anti rabiit IgG was used as the secondary. DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-HIF-2-alpha antibody [BL-95-1A2] (AB243861)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-HIF-2-alpha antibody [BL-95-1A2] (AB243861)

Formaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells untreated (Left) and treated with CoCl2 (right), labeling HIF-2 alpha (Red) using ab243861 at 1/100 dilution. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/100 dilution. Phalloidin Alexa Fluor® 488conjugated (green) was used as the counterstain.

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Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] (AB243861)

HIF-2 alpha was immunoprecipitated from 1 mg HepG2 whole cell lysate with ab243861 at 20 μL per reaction. Western blot was performed on the immunoprecipitate using ab243861 at 1/1000 dilution. Lane 1 : ab243861 IP in HepG2 cell lysate treated with 200 μM CoCl2.

Lane 2 : ab243861 IP in HepG2 whole cell lysate Mock treated.

Lane 3 : Contol IgG in HepG2 cell lysate treated with 200 μM CoCl2.

All lanes:

Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861)

Predicted band size: 96 kDa

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ChIP-sequencing - Anti-HIF-2-alpha antibody [BL-95-1A2] (AB243861)

ChIP-seq

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ChIP-sequencing - Anti-HIF-2-alpha antibody [BL-95-1A2] (AB243861)

Chromatin from subcutaneous human tumor 786-O cells was immunoprecipitated with anti-HIF-2-alpha antibody ab243861 and analyzed by
DNA sequencing. The figure illustrates the peak distribution of HIF-2-alpha binding within a 250 Kb region of chromosome 11 as detected
using anti-HIF-2-alpha antibody ab243861. ChIP-seq validation performed by Active Motif, Carlsbad, CA.

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Western blot - Anti-HIF-2-alpha antibody [BL-95-1A2] (AB243861)

All lanes:

Western blot - Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate treated with 200uM CoCl2 at 15 µg

Lane 2:

HepG2 whole cell lysate mock treated at 15 µg

Lane 3:

HEK293T whole cell lysate treated with 200uM CoCl2 at 15 µg

Lane 4:

HEK293T whole cell lysate mock treated at 15 µg

Lane 5:

786-O whole cell lysate mock treated at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 96 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

BL-95-1A2

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, ChIP-seq, IP, ICC, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human EPAS1 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

Q99814

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "Antigen retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker and overnight incubations are recommended. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/200 - 1/1000", "ICC-species-notes": "Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 5-20μl/mg lysate.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "10-40 µL for 30 µg chromatin", "ChIPseq-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-HIF-2-alpha antibody [BL-95-1A2] (ab243861) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Human samples.

Other related products
We have a range of other formats of antibody clone [BL-95-1A2] also available for your convenience: ab243861, ab272041

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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性能和储存信息

形式

Liquid

纯化说明

Recombinant antibody was purified from cell culture supernatant.

存储溶液

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HIF-2-alpha also known as hypoxia-inducible factor 2-alpha or EPAS1 plays a significant role in cellular response to oxygen levels. Mechanically HIF-2-alpha functions as a transcription factor that activates certain genes when oxygen is low. The protein typically weighs around 118 kDa. It is expressed in various tissues but particularly in endothelial cells and some neuronal tissues. These sites highlight its importance in organs requiring tight regulation of oxygen.

Biological function summary

HIF-2-alpha regulates the expression of genes involved in energy metabolism and angiogenesis. The protein forms a complex by dimerizing with the HIF-1-beta subunit which is necessary for transcriptional activity. Through this complex formation and activity it influences processes such as erythropoiesis and regulates factors like vascular endothelial growth factor (VEGF). Therefore HIF-2-alpha contributes to the adaptation of cells and tissues under hypoxic conditions.

Pathways

HIF-2-alpha engages in the hypoxia signaling pathway playing an essential part by modulating gene expression in response to low oxygen availability. This role impacts other proteins such as HIF-1-alpha sharing overlapping functions but with distinct target genes. Additionally HIF-2-alpha is involved in the mTOR pathway which influences cell growth and metabolism through its interaction with the nutrient-sensing regulatory pathway.

Researchers have linked HIF-2-alpha with conditions like renal cell carcinoma and pulmonary hypertension. The protein's dysregulation can lead to altered expression of angiogenic factors promoting tumor growth and survival. In particular its connection with VEGF exemplifies its role in these pathologies by facilitating aberrant blood vessel formation. Such interactions further highlight the potential of HIF-2-alpha as a therapeutic target in disease modulation.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD (By similarity).

See full target information EPAS1

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:312 PubMed40246814

2025

USP9X integrates TGF-β and hypoxia signalings to promote ovarian cancer chemoresistance via HIF-2α-maintained stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenlei Zhang,Xiujie Yu,Liqi Wen,Jia'nan Wang,Zhufeng Li,Yu Zhang,Jiayu Cheng,Ronglin Kan,Wanting Zhang,Yan Shen,Shukai Yuan,Li Zhao

Anticancer research 45:639-650 PubMed39890197

2025

Efficacy of Second-line Nivolumab Tyrosine Kinase Inhibitors for Renal Cell Carcinoma With Bone Metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Gaku Yamamichi,Taigo Kato,Akihiro Yoshimura,Masaru Tani,Yuki Horibe,Yutong Liu,Nesrine Sassi,Yohei Okuda,Toshiki Oka,Toshihiro Uemura,Akinaru Yamamoto,Y U Ishizuya,Takuji Hayashi,Yoshiyuki Yamamoto,Koji Hatano,Atsunari Kawashima,Tetsuya Takao,Kensaku Nishimura,Shingo Takada,Masao Tsujihata,Norio Nonomura

BJC reports 2:10 PubMed39516578

2024

Spatial functional mapping of hypoxia inducible factor heterodimerisation and immune checkpoint regulators in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Safrygina,Christopher Applebee,Alan McIntyre,Julian Padget,Banafshé Larijani

PLoS pathogens 20:e1011998 PubMed38530845

2024

Differential carbonic anhydrase activities control EBV-induced B-cell transformation and lytic cycle reactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Samaresh Malik,Joyanta Biswas,Purandar Sarkar,Subhadeep Nag,Chandrima Gain,Shatadru Ghosh Roy,Bireswar Bhattacharya,Dipanjan Ghosh,Abhik Saha

Oncology reports 51: PubMed38391012

2024

Effect of hypoxia‑HIF‑1α‑periostin axis in thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Yang,Junyi Wu,Huiqin Zhu,Xiaoqin Shi,Jun Liu,Yang Li,Min Wang

Journal of translational medicine 21:882 PubMed38057853

2023

GATA6-AS1 suppresses epithelial-mesenchymal transition of pancreatic cancer under hypoxia through regulating SNAI1 mRNA stability.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhui Zhou,Xinyi Zhou,Qiwen Ben,Ningning Liu,Jiahui Wang,Yongpeng Zhai,Yichen Bao,Lin Zhou

Journal of translational medicine 21:220 PubMed36967443

2023

PHF6 recruits BPTF to promote HIF-dependent pathway and progression in YAP-high breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Gao,Wensheng Zhang,Jingjing Ma,Xiaojian Ni

Anti-cancer agents in medicinal chemistry 23:807-816 PubMed36028958

2022

Embelin Enhances the Sensitivity of Renal Cancer Cells to Axitinib by Inhibiting HIF Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Fang,Zhiying Li,Ye Xue,Xin Zong,Wenshuang Ma,Guangmin Xi,Xiao Feng Zhang,Zuowei Li

Cell death & disease 13:463 PubMed35568711

2022

m6A methylation mediates LHPP acetylation as a tumour aerobic glycolysis suppressor to improve the prognosis of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Xian Lin,Ning-Zi Lian,You-Xin Gao,Qiao-Ling Zheng,Ying-Hong Yang,Yu-Bin Ma,Zhi-Song Xiu,Qing-Zhu Qiu,Hua-Gen Wang,Chao-Hui Zheng,Ping Li,Jian-Wei Xie,Jun Lu,Qi-Yue Chen,Long-Long Cao,Mi Lin,Jia-Bin Wang,Chang-Ming Huang

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34706936

2021

HIF-1-regulated expression of calreticulin promotes breast tumorigenesis and progression through Wnt/β-catenin pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxu Liu,Peiling Xie,Na Hao,Miao Zhang,Yang Liu,Peijun Liu,Gregg L Semenza,Jianjun He,Huimin Zhang

View all publications

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Anti-HIF-2-alpha 抗体 [BL-95-1A2]