Anti-HIF-1 alpha抗体(ab114977)
Key features and details
- Rabbit polyclonal to HIF-1 alpha
- Suitable for: IHC-P, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-HIF-1 alpha抗体
参阅全部 HIF-1 alpha 一抗 -
描述
兔多克隆抗体to HIF-1 alpha -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICCmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Carp, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human HIF-1 alpha aa 750-850 (C terminal).
Database link: Q16665 -
阳性对照
- IHC-P: Mouse squamous cell carcinoma tissue; ICC: HeLa cells.
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常规说明
Concentration is optimized for IHC and not determined.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
ab114977 was affinity purified using an epitope specific to HIF-1-alpha immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab114977于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) |
1/100 - 1/500. Epitope exposure is recommended.
Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.
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| ICC |
1/100 - 1/500.
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| 说明 |
|---|
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IHC-P
1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining. |
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ICC
1/100 - 1/500. |
靶标
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功能
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. -
组织特异性
Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
结构域
Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID). -
翻译后修饰
In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
Requires phosphorylation for DNA-binding.
Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.
Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia. - Information by UniProt
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数据库链接
- Entrez Gene: 374177 Chicken
- Entrez Gene: 281814 Cow
- Entrez Gene: 3091 Human
- Entrez Gene: 15251 Mouse
- Entrez Gene: 396696 Pig
- Entrez Gene: 100009579 Rabbit
- Entrez Gene: 29560 Rat
- Entrez Gene: 393202 Zebrafish
see all -
别名
- ARNT interacting protein antibody
- ARNT-interacting protein antibody
- Basic helix loop helix PAS protein MOP1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody (ab114977)
ab114977 at 1:250 dilution staining HIF-1-alpha in Formalin-fixed, Paraffin-embedded Mouse squamous cell carcinoma tissue by Immunohistochemistry.
Detection: DAB staining. -
Immunocytochemistry - Anti-HIF-1 alpha antibody (ab114977)Immunocytochemistry analysis of formaldehyde-fixed asynchronous hypoxic HeLa (Human cervix adenocarcinoma epithelial cell line) cells labeling HIF-1 alpha with ab114977 at a 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab114977 被引用在 23 文献中.
- Wu J et al. Oxygen microcapsules improve immune checkpoint blockade by ameliorating hypoxia condition in pancreatic ductal adenocarcinoma. Bioact Mater 20:259-270 (2023). PubMed: 35702611
- Chen J et al. α‑hederin overcomes hypoxia‑mediated drug resistance in colorectal cancer by inhibiting the AKT/Bcl2 pathway. Int J Oncol 62:N/A (2023). PubMed: 36704835
- Ji S et al. A Dual-Crosslinked Hydrogel Based on Gelatin Methacryloyl and Sulfhydrylated Chitosan for Promoting Wound Healing. Int J Mol Sci 24:N/A (2023). PubMed: 36768768
- Zhang H et al. METTL3-mediated N6-methyladenosine exacerbates ferroptosis via m6A-IGF2BP2-dependent mitochondrial metabolic reprogramming in sepsis-induced acute lung injury. Clin Transl Med 13:e1389 (2023). PubMed: 37715457
- Shimoji K et al. Hypoxia-inducible factor 1α modulates interstitial pneumonia-mediated lung cancer progression. J Transl Med 21:857 (2023). PubMed: 38012636