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使用敲除细胞株进行验证重组RabMAb

重组Anti-HIBADH抗体[EPR12519(B)] (ab175203)

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Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
  • Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
  • Flow Cytometry (Intracellular) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
  • Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
  • Immunoprecipitation - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
  • Anti-HIBADH antibody [EPR12519(B)] (ab175203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12519(B)] to HIBADH
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-HIBADH抗体[EPR12519(B)]
    参阅全部 HIBADH 一抗

  • 描述

    兔单克隆抗体[EPR12519(B)] to HIBADH

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Cow, Orangutan不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human HIBADH aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P31937

  • 阳性对照

    • WB: HeLa and HepG2 cell lysates; Human fetal liver, fetal heart and fetal kidney tissue lysates. IHC-P: Human kidney and liver tissue. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab175203于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.

靶标

图片

  • Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
    Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
    All lanes : Anti-HIBADH antibody [EPR12519(B)] (ab175203) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : HIBADH knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab175203 observed at 35 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab175203 Anti-HIBADH antibody [EPR12519(B)] was shown to specifically react with HIBADH in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265888 (knockout cell lysate ab257986) was used. Wild-type and HIBADH knockout samples were subjected to SDS-PAGE. ab175203 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
    Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

    Immunofluorescent staining of HepG2 cells labeling HIBADH with ab175203 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling HIBADH with ab175203 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
    Flow Cytometry (Intracellular) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

    Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling HIBADHwith ab175203at 1/10 dilution (red) compared to a Rabbit IgG (green). 

     

     

  • Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
    Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
    All lanes : Anti-HIBADH antibody [EPR12519(B)] (ab175203) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labeling HIBADH with ab175203 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
    Immunoprecipitation - Anti-HIBADH antibody [EPR12519(B)] (ab175203)

    Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate labeling HIBADH with ab175203 at 1/10 dilution.

  • Anti-HIBADH antibody [EPR12519(B)] (ab175203)
    Anti-HIBADH antibody [EPR12519(B)] (ab175203)

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