Anti-hHR23A抗体(ab3836)
Key features and details
- Goat polyclonal to hHR23A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-hHR23A抗体
参阅全部 hHR23A 一抗 -
描述
山羊多克隆抗体to hHR23A -
宿主
Goat -
特异性
This antibody has been shown to detect an approxiametely 60kd band in HeLa and Jurkat lysates. This is comparable in size to the band detected in HeLa in van der Spek et al. 1996. We believe this is good evidence that this antibody is detecting hHR23A.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide:
KSPSEESAPTTSPEC
, corresponding to amino acids 121-133 of Human hHR23A. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 40 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 40 kDa). |
靶标
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功能
Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
Involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome. -
序列相似性
Belongs to the RAD23 family.
Contains 2 UBA domains.
Contains 1 ubiquitin-like domain. -
结构域
The ubiquitin-like domain mediates interaction with ATXN3.
The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5886 Human
- Entrez Gene: 19358 Mouse
- Omim: 600061 Human
- SwissProt: P54725 Human
- SwissProt: P54726 Mouse
- Unigene: 643267 Human
- Unigene: 485055 Mouse
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别名
- hHR 23A antibody
- hHR23A antibody
- HR23A antibody
see all
图片
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Western blot - Anti-hHR23A antibody (ab3836)
Western blot using ab3836 at 1/500.
Secondary ab: Rabbit anti Goat ab6741 1/5000
Exposure time:30 secondsLane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab3836 尚未被引用在任何文献中。