Anti-Hhip抗体(ab39208)
Key features and details
- Rabbit polyclonal to Hhip
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Hhip抗体
参阅全部 Hhip 一抗 -
描述
兔多克隆抗体to Hhip -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Hhip.
参阅Abcam的专有抗源政策 (Peptide available as ab39947.) -
阳性对照
- This antibody gave a positive signal in the following lysates: HeLa Whole Cell; Mouse Heart; Mouse Liver; Mouse Pancreas.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39208于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79 kDa). |
靶标
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功能
Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family. -
组织特异性
Widely expressed in fetal and adult tissues. Highest expression in adult heart, liver and pancreas, and in fetal kidney. -
序列相似性
Belongs to the HHIP family.
Contains 2 EGF-like domains. -
结构域
A flexible loop interacts with the SHH zinc binding site and contributes to zinc binding. -
细胞定位
Cytoplasm and Cell membrane. Secreted. The last 22 C-terminal amino acids may participate in cell membrane attachment. - Information by UniProt
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数据库链接
- Entrez Gene: 540830 Cow
- Entrez Gene: 64399 Human
- Entrez Gene: 15245 Mouse
- Omim: 606178 Human
- SwissProt: Q96QV1 Human
- SwissProt: Q7TN16 Mouse
- Unigene: 507991 Human
- Unigene: 254493 Mouse
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别名
- FLJ20992 antibody
- FLJ90230 antibody
- Hedgehog interacting protein antibody
see all
图片
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Western blot - Anti-Hhip antibody (ab39208)All lanes : Anti-Hhip antibody (ab39208) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 : Pancreas (Mouse) Tissue Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 6 : Heart (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 7 : Liver (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lane 8 : Pancreas (Mouse) Tissue Lysate with Human Hhip peptide (ab39947) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 45 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab39208 被引用在 5 文献中.
- Qiu S et al. LncRNA EGOT decreases breast cancer cell viability and migration via inactivation of the Hedgehog pathway. FEBS Open Bio 10:817-826 (2020). PubMed: 32150666
- Lee BNR et al. Hedgehog-Interacting Protein (HIP) Regulates Apoptosis Evasion and Angiogenic Function of Late Endothelial Progenitor Cells. Sci Rep 7:12449 (2017). WB ; Mouse . PubMed: 28963460
- Wang C et al. EZH2 Mediates epigenetic silencing of neuroblastoma suppressor genes CASZ1, CLU, RUNX3, and NGFR. Cancer Res 72:315-24 (2012). PubMed: 22068036
- Zhou X et al. Identification of a chronic obstructive pulmonary disease genetic determinant that regulates HHIP. Hum Mol Genet 21:1325-35 (2012). WB ; Human . PubMed: 22140090
- Angeloni NL et al. The role of hedgehog-interacting protein in maintaining cavernous nerve integrity and adult penile morphology. J Sex Med 6:2480-93 (2009). IHC-Fr ; Rat . PubMed: 19515211