为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Signal Transduction Growth Factors/Hormones Hormones

Anti-HGF抗体(ab24865)

  • Datasheet
Reviews (2)Q&A (1)References (8)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-HGF antibody (ab24865)
  • Sandwich ELISA - Anti-HGF antibody (ab24865)

Key features and details

  • Rabbit polyclonal to HGF
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Human
  • Isotype: IgG

你可能会感兴趣

一抗
Product image
Anti-NeuroD2 antibody [EPR5135] (ab109406)
Western blot
Product image
InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
一抗
Product image
Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab192489)

查看更多相关产品

概述

  • 产品名称

    Anti-HGF抗体
    参阅全部 HGF 一抗
  • 描述

    兔多克隆抗体to HGF
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, Sandwich ELISAmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Recombinant full length HGF (Human)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液

    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human HGF protein (Active) (ab632)
  • sELISA pair antibody

    • Anti-HGF antibody [EGH2 4C12.1] (ab10829)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab24865于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83 kDa.
Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829).

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab10829 as Capture.

说明
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83 kDa.
Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829).

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab10829 as Capture.

靶标

  • 功能

    Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • 疾病相关

    Deafness, autosomal recessive, 39
  • 序列相似性

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Target information above from: UniProt accession P14210 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3082 Human
    • Omim: 142409 Human
    • SwissProt: P14210 Human
    • Unigene: 396530 Human
    • Unigene: 561679 Human
    • 别名

      • DFNB39 antibody
      • F TCF antibody
      • Fibroblast derived tumor cytotoxic factor antibody
      • Hepatocyte growth factor (hepapoietin A; scatter factor) antibody
      • Hepatocyte growth factor antibody
      • Hepatocyte growth factor beta chain antibody
      • Hepatocyte growth factor precursor antibody
      • Hepatopoietin A antibody
      • Hepatopoietin-A antibody
      • Hgf antibody
      • HGF_HUMAN antibody
      • HGFB antibody
      • HPTA antibody
      • Lung fibroblast derived mitogen antibody
      • OTTHUMP00000161349 antibody
      • OTTHUMP00000206710 antibody
      • OTTHUMP00000206711 antibody
      • OTTHUMP00000206712 antibody
      • OTTHUMP00000206713 antibody
      • OTTHUMP00000206730 antibody
      • Scatter factor antibody
      • SF antibody
      see all

    图片

    • Western blot - Anti-HGF antibody (ab24865)
      Western blot - Anti-HGF antibody (ab24865)
      Anti-HGF antibody (ab24865) at 1 µg/ml + Recombinant human HGF protein (ab105061) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 83 kDa


      Exposure time: 1 minute
    • Sandwich ELISA - Anti-HGF antibody (ab24865)
      Sandwich ELISA - Anti-HGF antibody (ab24865)

      Standard Curve for HGF (Analyte: ab632) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829) at 5ug/ml and Detector Antibody Rabbit polyclonal to HGF (ab24865) at 0.5ug/ml.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (8)

    发表研究结果有使用 ab24865?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab24865 被引用在 8 文献中.

    • Kim YJ  et al. Area light source-triggered latent angiogenic molecular mechanisms intensify therapeutic efficacy of adult stem cells. Bioeng Transl Med 7:e10255 (2022). PubMed: 35079630
    • Du T  et al. Microvesicles derived from human umbilical cord mesenchyme promote M2 macrophage polarization and ameliorate renal fibrosis following partial nephrectomy via hepatocyte growth factor. Hum Cell 34:1103-1113 (2021). PubMed: 33860459
    • Wen Q  et al. 8-bromo-7-methoxychrysin suppress stemness of SMMC-7721 cells induced by co-culture of liver cancer stem-like cells with hepatic stellate cells. BMC Cancer 19:224 (2019). PubMed: 30866863
    • Kogata Y  et al. Foretinib (GSK1363089) induces p53-dependent apoptosis in endometrial cancer. Oncotarget 9:22769-22784 (2018). PubMed: 29854314
    • Cao XF  et al. Therapeutic effects of hepatocyte growth factor-overexpressing dental pulp stem cells on liver cirrhosis in a rat model. Sci Rep 7:15812 (2017). PubMed: 29150644
    • Ono YJ  et al. Estradiol-mediated hepatocyte growth factor is involved in the implantation of endometriotic cells via the mesothelial-to-mesenchymal transition in the peritoneum. Am J Physiol Endocrinol Metab 308:E950-9 (2015). PubMed: 25852006
    • Cohen ME  et al. Signaling through three chemokine receptors triggers the migration of transplanted neural precursor cells in a model of multiple sclerosis. Stem Cell Res 13:227-39 (2014). IHC-FoFr ; Mouse . PubMed: 25086214
    • Goldoni S  et al. Decorin is a novel antagonistic ligand of the Met receptor. J Cell Biol 185:743-54 (2009). WB ; Human . PubMed: 19433454

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HGF antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil,cerebellum,fetal liver,normal breast,fetal)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6.0
    Permeabilization
    Yes - 0.05%Tween20 in PBS
    Specification
    Tonsil,cerebellum,fetal liver,normal breast,fetal
    Blocking step
    sea block (fisher#37527) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 28 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HGF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human mesenchymal stem cells transduced with Adv-H)
    Specification
    Human mesenchymal stem cells transduced with Adv-H
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Hao Wu

    Verified customer

    提交于 Dec 03 2010

    Question

    Will this antibody recognize the inactive & active form of the protein?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 07 2012

    Answer

    Thank you for contacting Abcam.

    The antibody was generated against the active recombinant protein of HGF, so it should also recognize the inactive precursor form. However we do not have the possibility to check it.

    Also, there is a 90% level of homology between the human sequence and mouse, therefore there is strong chance that the antibody will work in this species. As ab24865 has not been tested in mouse samples, you can participate in our AbTrial program. Simply follow these easy steps below to apply for our AbTrial Program:

    1. Reply to this email, letting us know you are interested in testing this product.

    2. Our scientists will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at the regular price.

    4. Submit your results, including your discount code in the additional notes section of your Abreview.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

    Read More

    Abcam Scientific Support

    回复于 Nov 07 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2025 艾博抗(上海)贸易有限公司版权所有