重组Anti-HGD抗体[EPR7874] (ab131035)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7874] to HGD
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HGD抗体[EPR7874]
参阅全部 HGD 一抗 -
描述
兔单克隆抗体[EPR7874] to HGD -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human HGD aa 400-500 (C terminal). The exact sequence is proprietary.
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阳性对照
- Caco-2, HepG2, and Human fetal liver lysates; Human kidney tissue; HepG2 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7874 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131035于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
靶标
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组织特异性
Highest expression in the prostate, small intestine, colon, kidney and liver. -
通路
Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. -
疾病相关
Defects in HGD are the cause of alkaptonuria (AKU) [MIM:203500]. AKU is an autosomal recessive error of metabolism characterized by an increase in the level of homogentisic acid. The clinical manifestations of AKU are urine that turns dark on standing and alkalinization, black ochronotic pigmentation of cartilage and collagenous tissues, and spine arthritis. -
序列相似性
Belongs to the homogentisate dioxygenase family. - Information by UniProt
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数据库链接
- Entrez Gene: 3081 Human
- Omim: 607474 Human
- SwissProt: Q93099 Human
- Unigene: 368254 Human
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别名
- 2-dioxygenase antibody
- AKU antibody
- FLJ94126 antibody
see all
图片
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All lanes : Anti-HGD antibody [EPR7874] (ab131035) at 1/1000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody [EPR7874] (ab131035)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HGD with ab131035 antibody at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of HepG2 cells labelling HGD with ab131035 antibody at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab131035 被引用在 2 文献中.
- Chen XT et al. Non-invasive urinary sediment double-immunostaining predicts BK polyomavirus associated-nephropathy in kidney transplant recipients. Ann Transl Med 8:235 (2020). PubMed: 32309382
- Huang Y et al. Detection of Proximal Tubule Involvement by BK Polyomavirus in Kidney Transplant Recipients With Urinary Sediment Double-Immunostaining. Front Immunol 11:582678 (2020). PubMed: 33072129