重组Anti-HFE抗体[EPR6751(2)]

• WB

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Western blot - Anti-HFE antibody [EPR6751(2)] (AB133639)

All lanes:

Western blot - Anti-HFE antibody [EPR6751(2)] (ab133639) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

false

• WB

AbReview45186****

Western blot - Anti-HFE antibody [EPR6751(2)] (AB133639)

Lanes 1 - 2:

Western blot - Anti-HFE antibody [EPR6751(2)] (ab133639) at 1/1000 dilution

Lanes 3 - 4:

Rabbit monoclonal anti-myc tag

Lanes 1 and 3:

HepG2 whole cell lysate - untransfected at 25 µg

Lanes 2 and 4:

HepG2 whole cell lysate - transfected with pCMV6 human HFE Tag Flag Myc at 25 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa,60 kDa

true

Exposure time: 5s

This image is courtesy of an Abreview submitted by Chloe Latour

• OI-RD Scanning

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OI-RD Scanning - Anti-HFE antibody [EPR6751(2)] (AB133639)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.