Anti-Hexokinase 1抗体- Mitochondrial Outer膜Marker (ab65069)
Key features and details
- Rabbit polyclonal to Hexokinase 1 - Mitochondrial Outer Membrane Marker
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Hexokinase 1抗体- Mitochondrial Outer膜Marker
参阅全部 Hexokinase 1 一抗 -
描述
兔多克隆抗体to Hexokinase 1 - Mitochondrial Outer膜Marker -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide derived from an internal sequence of human Hexokinase 1. [Swiss-Prot P19367]
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65069于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). |
靶标
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组织特异性
Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific. -
通路
Carbohydrate metabolism; hexose metabolism. -
疾病相关
Hexokinase deficiency
Neuropathy, hereditary motor and sensory, Russe type -
序列相似性
Belongs to the hexokinase family.
Contains 2 hexokinase domains. -
结构域
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. -
细胞定位
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. - Information by UniProt
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数据库链接
- Entrez Gene: 3098 Human
- Omim: 142600 Human
- SwissProt: P19367 Human
- Unigene: 370365 Human
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别名
- BB404130 antibody
- Brain form hexokinase antibody
- dea antibody
see all
图片
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Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069)All lanes : Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from HeLa cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069)Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069) at 1/500 dilution +Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Exposure time: 4 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab65069 被引用在 5 文献中.
- Li J et al. Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules. JCI Insight 5:N/A (2020). PubMed: 32134397
- Sheikh MH et al. Immuno-metabolic impact of the multiple sclerosis patients' sera on endothelial cells of the blood-brain barrier. J Neuroinflammation 17:153 (2020). PubMed: 32386505
- Yu L et al. Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism. J Exp Clin Cancer Res 36:110 (2017). PubMed: 28810896
- Kishore M et al. Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis. Immunity 47:875-889.e10 (2017). PubMed: 29166588
- Tegnebratt T et al. Evaluation of efficacy of a new MEK inhibitor, RO4987655, in human tumor xenografts by [(18)F] FDG-PET imaging combined with proteomic approaches. EJNMMI Res 4:34 (2014). IHC . PubMed: 26116108