重组Anti-Hexokinase 1抗体[EPR10134(B)] –小鼠IgG1 (Chimeric) – BSA and Azide Free (ab317798)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317798)](https://www.abcam.cn/ps/products/317/ab317798/Images/ab317798-541580-anti-hexokinase-1-antibody-epr10134b-mitochondrial-omm-mouse-igg1-chimeric-immunohistochemistry-stomach-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317798)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [EPR10134(B)] to Hexokinase 1 - Chimeric – BSA and Azide free
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Hexokinase 1抗体[EPR10134(B)] –小鼠IgG1 (Chimeric) – BSA and Azide Free
参阅全部 Hexokinase 1 一抗 -
描述
小鼠单克隆抗体[EPR10134(B)] to Hexokinase 1 - Chimeric – BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human stomach tissue. ICC/IF: MCF7 cells.
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常规说明
AB317798 is the carrier-free version of AB317788.
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab150423). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
EPR10134(B) -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab150423)
- Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab184818)
- Alexa Fluor® 647 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab197864)
- Anti-Hexokinase 1 antibody [EPR10134(B)] - BSA and Azide free (ab233837)
- PE Anti-Hexokinase 1 - Mitochondrial Outer Membrane Marker antibody [EPR10134(B)] (ab303119)
- APC Anti-Hexokinase 1 - Mitochondrial Outer Membrane Marker antibody [EPR10134(B)] (ab303120)
- HRP Anti-Hexokinase 1 - Mitochondrial Outer Membrane Marker antibody [EPR10134(B)] (ab303121)
- AP Anti-Hexokinase 1 antibody [EPR10134(B)] (ab308989)
- Alexa Fluor® 594 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab310762)
- Alexa Fluor® 555 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab312289)
- Alexa Fluor® 568 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab312784)
- Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial OMM – Mouse IgG1 (Chimeric) (ab317788)
- Alexa Fluor® 750 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker (ab321126)
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317798于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/500.
|
| 说明 |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/500. |
靶标
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组织特异性
Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific. -
通路
Carbohydrate metabolism; hexose metabolism. -
疾病相关
Hexokinase deficiency
Neuropathy, hereditary motor and sensory, Russe type -
序列相似性
Belongs to the hexokinase family.
Contains 2 hexokinase domains. -
结构域
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. -
细胞定位
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. - Information by UniProt
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数据库链接
- Entrez Gene: 3098 Human
- Omim: 142600 Human
- SwissProt: P19367 Human
- Unigene: 370365 Human
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别名
- BB404130 antibody
- Brain form hexokinase antibody
- dea antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317798)
Immunofluorescence staining of hexokinase 1 in sections of formalin-fixed paraffin-embedded human stomach (positive) and human pancreas (negative).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317798 at 1/100 dilution, and then incubated for 1 hour with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317798)](https://www.abcam.cn/ps/products/317/ab317798/Images/ab317798-541578-anti-hexokinase-1-antibody-epr10134b-mitochondrial-omm-mouse-igg1-chimeric-immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317798)This image was produced using ab317798, the same antibody clone but in a different formulation.
ab317798 staining Hexokinase-1 in MCF7 (positive) and HepG2 (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab317798 at 1µg/ml and ab185031, Alexa Fluor® 488 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker. Cells were then incubated with ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317798)](https://www.abcam.cn/ps/products/317/ab317798/Images/ab317798-542320-BenefitsofRecombinantantibodies.jpg)
Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317798)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab317798 尚未被引用在任何文献中。
