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重组RabMAb

重组Anti-HEXIM1抗体[EPR23430-12] - BSA and Azide free (ab272694)

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Immunoprecipitation - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
  • Immunoprecipitation - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
  • Immunocytochemistry/ Immunofluorescence - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
  • ChIP - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
  • Flow Cytometry (Intracellular) - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
  • ChIP - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
  • Immunocytochemistry/ Immunofluorescence - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
  • Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23430-12] to HEXIM1 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt (Intra), IP, IHC-P, ChIP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-HEXIM1抗体[EPR23430-12] - BSA and Azide free
    参阅全部 HEXIM1 一抗

  • 描述

    兔单克隆抗体[EPR23430-12] to HEXIM1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, Flow Cyt (Intra), IP, IHC-P, ChIP, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: LNCaP, PC-12 whole cell lysates. IHC-P: Human cardiac muscle and testis tissue. ICC/IF: HeLa and MEF cells. Flow Cyt (intra): HeLa cells. IP: HeLa (treated with 10 M MG-132 for 24 hours) and MEF whole cell lysates.

  • 常规说明

    ab272694 is the carrier-free version of ab240647.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272694于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 41 kDa).
说明
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 41 kDa).

靶标

  • 功能

    Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.

  • 组织特异性

    Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.

  • 序列相似性

    Belongs to the HEXIM family.

  • 结构域

    The coiled-coil domain mediates oligomerization.

  • 细胞定位

    Nucleus. Cytoplasm. Binds alpha-importin and is mostly nuclear.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Cardiac lineage protein 1 antibody
    • CLP 1 antibody
    • CLP1 antibody
    • EDG 1 antibody
    • EDG1 antibody
    • Estrogen down-regulated gene 1 protein antibody
    • FLJ13562 antibody
    • Hexamethylene bis acetamide inducible 1 antibody
    • Hexamethylene bis acetamide inducible protein antibody
    • Hexamethylene bis acetamide inducible transcript 1 antibody
    • Hexamethylene bis-acetamide-inducible protein 1 antibody
    • HEXI1_HUMAN antibody
    • HEXIM 1 antibody
    • Hexim1 antibody
    • HEXIM1 protein antibody
    • HIS 1 antibody
    • HIS1 antibody
    • HMBA inducible antibody
    • MAQ 1 antibody
    • MAQ1 antibody
    • Menage a quatre 1 antibody
    • Menage a quatre protein 1 antibody
    • Protein HEXIM1 antibody
    see all

图片

  • Immunoprecipitation - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
    Immunoprecipitation - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)

    HEXIM1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) (treated with 10 μM MG-132 for 24 hours), whole cell lysate with ab240647 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240647 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) treated with 10 μM MG-132 for 24 hours, whole cell lysate 10 ug

    Lane 2: ab240647 IP in HeLa treated with 10 μM MG-132 for 24 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240647 in HeLa treated with 10 μM MG-132 for 24 hours whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240647).

  • Immunoprecipitation - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
    Immunoprecipitation - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)

    HEXIM1 was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate with ab240647 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240647 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate 10 ug

    Lane 2: ab240647 IP in MEF whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240647 in MEF whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Lysate were made freshly and used in IP test immediately to minimize protein degradation. Incubation for immunoprecipitation was carried out overnight at 4°C.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240647).

  • Immunocytochemistry/ Immunofluorescence - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
    Immunocytochemistry/ Immunofluorescence - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)

    Immunofluorescent analysis of 100% methanol-fixed MEF cells labelling HEXIM1 with ab240647 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000  (Green). Confocal image showing nuclear staining in MEFs. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240647).

  • ChIP - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
    ChIP - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)

    Chromatin was prepared from MEF cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab 240647 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
    Primers and probes are from paper PMCID: PMC4103662

    *https://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240647).

  • Flow Cytometry (Intracellular) - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
    Flow Cytometry (Intracellular) - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling HEXIM1 with ab240647 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

    A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240647). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling HEXIM1 with ab240647 at 1/2000 dilution  dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis (PMID: 23300697). The section was incubated with ab240647 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240647).

  • ChIP - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
    ChIP - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)

    Chromatin was prepared from LNCaP cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab240647 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).

    https://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240647). 

  • Immunocytochemistry/ Immunofluorescence - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
    Immunocytochemistry/ Immunofluorescence - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)

    Immunofluorescent analysis of 100% methanol-fixed HeLa cells labelling HEXIM1 with ab240647 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000  (Green). Confocal image showing nuclear staining in HeLa cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200  dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240647).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling HEXIM1 with ab240647 at 1/2000 dilution dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human cardiac muscle (PMID: 23300697). The section was incubated with ab240647 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240647).

  • Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)
    Anti-HEXIM1 antibody [EPR23430-12] - BSA and Azide free (ab272694)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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