Anti-HEXIM1抗体(ab58573)
Key features and details
- Goat polyclonal to HEXIM1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-HEXIM1抗体
参阅全部 HEXIM1 一抗 -
描述
山羊多克隆抗体to HEXIM1 -
宿主
Goat -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow
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免疫原
Synthetic peptide corresponding to Human HEXIM1 aa 346-359 (C terminal).
Sequence:C-HRQQERAPLSKFGD
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阳性对照
- WB: Human and mouse heart tissue lysates. ICC/IF: HeLa and A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58573于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
1 hour primary incubation is recommended for this product. |
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| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). 1 hour primary incubation is recommended for this product. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. -
组织特异性
Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. -
序列相似性
Belongs to the HEXIM family. -
结构域
The coiled-coil domain mediates oligomerization. -
细胞定位
Nucleus. Cytoplasm. Binds alpha-importin and is mostly nuclear. - Information by UniProt
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数据库链接
- Entrez Gene: 10614 Human
- Entrez Gene: 192231 Mouse
- Entrez Gene: 498008 Rat
- Omim: 607328 Human
- SwissProt: O94992 Human
- SwissProt: Q8R409 Mouse
- SwissProt: Q5M9G1 Rat
- Unigene: 15299 Human
see all -
别名
- Cardiac lineage protein 1 antibody
- CLP 1 antibody
- CLP1 antibody
see all
图片
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Western blot - Anti-HEXIM1 antibody (ab58573)All lanes : Anti-HEXIM1 antibody (ab58573) at 0.1 µg/ml
Lane 1 : Human heart tissue lysate in RIPA buffer
Lane 2 : Mouse heart tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDaIncubated with the primary antibody for 1 hour.
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Immunocytochemistry/ Immunofluorescence - Anti-HEXIM1 antibody (ab58573)Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation for 1hr with ab58573 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-HEXIM1 antibody (ab58573)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation for 1hr with ab58573 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
数据表及文件
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Datasheet download
文献 (0)
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