重组Anti-Hes1抗体[EPR4226] (ab108937)
![Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [EPR4226] (ab108937)](https://www.abcam.cn/ps/products/108/ab108937/Images/ab108937-484403-anti-hes1-antibody-epr4226-immunocytochemistry-immunofluorescence-sh-sy5y-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [EPR4226] (ab108937)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4226] to Hes1
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Hes1抗体[EPR4226]
参阅全部 Hes1 一抗 -
描述
兔单克隆抗体[EPR4226] to Hes1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details
不适用于: IP -
种属反应性
与反应: Rat, Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SH-SY5Y, MOLT-4, MDA-MB-231, MCF7, K-562, HUVEC, HepG2, Human brain, Human liver, Human placenta.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4226 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108937于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
1/1000.
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| WB | (4) |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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| IHC-P | (5) |
1/90 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
1/20 - 1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
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ICC/IF
1/1000. |
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WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
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IHC-P
1/90 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/20 - 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
结构域
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3280 Human
- Entrez Gene: 15205 Mouse
- Entrez Gene: 29577 Rat
- Omim: 139605 Human
- SwissProt: Q14469 Human
- SwissProt: P35428 Mouse
- SwissProt: Q04666 Rat
- Unigene: 250666 Human
see all -
别名
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [EPR4226] (ab108937)
Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% TritonX-100 permeabilised SH-SY5Y (Human neuroblastoma epithelial cell) labelling Hes1 with ab108937 at 1/1000 dilution, followed by AlexaFluor® 488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in SH-SY5Y cell line is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain alpha Tubulin at 1/200 dilution (red). The Nuclear counterstain was DAPI (blue).
Secondary antibody only control: Secondary antibody is AlexaFluor® 488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
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![Western blot - Anti-Hes1 antibody [EPR4226] (ab108937)](https://www.abcam.cn/ps/products/108/ab108937/Images/ab108937-483489-anti-hes1-antibody-epr4226-western-blot-sh-sy5y-molt-4-mda-mb-231-mcf7-k-562-huvec-hepg2-brain-liver-tissue-lysate-human.jpg)
Western blot - Anti-Hes1 antibody [EPR4226] (ab108937)All lanes : Anti-Hes1 antibody [EPR4226] (ab108937) at 1/1000 dilution
Lane 1 : SH-SY5Y (Human bone marrow epithelial) whole cell lysate
Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 6 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
Lane 7 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 8 : Human brain tissue lysate
Lane 9 : Human liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 120 secondsBlocking and diluting buffer and concentration: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] (ab108937)](https://www.abcam.cn/ps/products/108/ab108937/Images/ab108937-239405-anti-hes1-antibody-epr4226-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] (ab108937)Primary ab concentration dilution: 1:200, (0.5ug/ml)
Secondary ab: ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG
Secondary ab concentration: Prediluted
Tissue: Human brain
Fixative: Paraffin-embedded sections
Counter stain: Hematoxylin
Antigen retrieval: Perform heat mediated antigen retrieval using Tris/EDTA Buffer, PH9
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![Flow Cytometry (Intracellular) - Anti-Hes1 antibody [EPR4226] (ab108937)](https://www.abcam.cn/ps/products/108/ab108937/Images/ab108937-121438-anti-hes1-antibody-epr4226-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Hes1 antibody [EPR4226] (ab108937)Overlay histogram showing SH-SY5Y cells stained with unpurifiedab108937 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108937, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] (ab108937)](https://www.abcam.cn/ps/products/108/ab108937/Images/ab108937-121439-anti-hes1-antibody-epr4226-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] (ab108937)Immunohistochemical staining of Hes1 in paraffin-embedded human placenta tissue with unpurified ab108937 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] (ab108937)](https://www.abcam.cn/ps/products/108/ab108937/Images/ab108937-239404-anti-hes1-antibody-epr4226-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] (ab108937)Immunohistochemical staining of paraffin embedded human brain with unpurified ab108937 at a working dilution of 1 in 90. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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![Anti-Hes1 antibody [EPR4226] (ab108937)](https://www.abcam.cn/ps/products/108/ab108937/Images/ab108937-12-benefits-of-recombinant-antibodies.png)
Anti-Hes1 antibody [EPR4226] (ab108937)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (82)
ab108937 被引用在 82 文献中.
- Zhang W et al. Single-cell sequencing reveals SATB2/NOTCH1 signaling promotes the progression of malignancy of epithelial cells from papillary thyroid cancer. Mol Carcinog 63:22-33 (2024). PubMed: 37877736
- Zhao E et al. NBP Cytoprotective Effects Promoting Neuronal Differentiation in BMSCs by Inhibiting the p65/Hes1 Pathway. Iran J Pharm Res 22:e132496 (2023). PubMed: 38116559
- Chai H et al. Histone methyltransferase SETD1A interacts with notch and promotes notch transactivation to augment ovarian cancer development. BMC Cancer 23:96 (2023). PubMed: 36707804
- Jiang W et al. A NOTCH1 Mutation Found in a Newly Established Ovarian Cancer Cell Line (FDOVL) Promotes Lymph Node Metastasis in Ovarian Cancer. Int J Mol Sci 24:N/A (2023). PubMed: 36982170
- Zhang Y et al. Di-(2-ethylhexyl) phthalate (DEHP) promoted hepatic lipid accumulation by activating Notch signaling pathway. Environ Toxicol 38:1628-1640 (2023). PubMed: 36988346