Anti-Hepcidin-25抗体(ab30760)
Key features and details
- Rabbit polyclonal to Hepcidin-25
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Hepcidin-25抗体
参阅全部 Hepcidin-25 一抗 -
描述
兔多克隆抗体to Hepcidin-25 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all species. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Hepcidin-25.
参阅Abcam的专有抗源政策 (Peptide available as ab31875.) -
阳性对照
- WB: Recombinant Hepcidin protein. IHC-P: Human liver tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab30760于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (5) |
1/10 - 1/100. Detects a band of approximately 32 kDa (predicted molecular weight: 3 kDa).
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| IHC-P | (1) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/10 - 1/100. Detects a band of approximately 32 kDa (predicted molecular weight: 3 kDa). |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317). -
组织特异性
Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine. -
疾病相关
Hemochromatosis 2B -
序列相似性
Belongs to the hepcidin family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 57817 Human
- Entrez Gene: 84506 Mouse
- Entrez Gene: 84604 Rat
- Omim: 606464 Human
- SwissProt: P81172 Human
- SwissProt: Q9EQ21 Mouse
- SwissProt: Q99MH3 Rat
- Unigene: 8821 Human
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形式
Hepcidin produces a propeptide of 84 amino acids that undergoes enzymatic cleavage into mature peptides of 20, 22, and 25 amino acids. Active peptides are rich in cysteines that form intramolecular bonds and stabilize the beta-sheet structure -
别名
- Hamp antibody
- HEPC antibody
- HEPC_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760)
IHC image of Hepcidin-25 staining in Human normal liver formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30760, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Anti-Hepcidin-25 antibody (ab30760)Anti-Hepcidin-25 antibody (ab30760) at 1 µg/ml + HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 3 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab30760 was tested against Human HAMP Full-length Recombinant Protein (Tagged) predicted to run at 32kDa. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760)This image is courtesy of Ruma Raha-Chowdhury (Cambridge University)Hepcidin-25 expression in rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760). Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (41)
ab30760 被引用在 41 文献中.
- Hitomi S et al. Hepcidin expression in the trigeminal ganglion and the oral mucosa in an oral ulcerative mucositis rat model. PLoS One 18:e0284617 (2023). PubMed: 37079608
- Lv H et al. A static magnetic field improves bone quality and balances the function of bone cells with regulation on iron metabolism and redox status in type 1 diabetes. FASEB J 37:e22985 (2023). PubMed: 37249350
- Wang D et al. Loss of SLC46A1 decreases tumor iron content in hepatocellular carcinoma. Hepatol Commun 6:2914-2924 (2022). PubMed: 35811443
- Kaushik SR et al. Low circulatory Fe and Se levels with a higher IL-6/IL-10 ratio provide nutritional immunity in tuberculosis. Front Immunol 13:985538 (2022). PubMed: 36713405
- Che J et al. PTH 1-34 promoted bone formation by regulating iron metabolism in unloading-induced bone loss. Front Endocrinol (Lausanne) 13:1048818 (2022). PubMed: 36818465