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AB13833

Anti-Hepatitis C Virus NS5a 抗体 [H26]

Anti-Hepatitis C Virus NS5a antibody [H26]

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(10 Publications)

Mouse Monoclonal Hepatitis C Virus NS5a antibody. Suitable for ELISA, WB, ICC/IF and reacts with Hepatitis C virus samples. Cited in 10 publications.

查看别名

HCV, HCV Non-structural protein 5A, NS5A, Non-structural protein 5A, p56

1 Images
Western blot - Anti-Hepatitis C Virus NS5a antibody [H26] (AB13833)
  • WB

PubMed

Western blot - Anti-Hepatitis C Virus NS5a antibody [H26] (AB13833)

Representative western blot detecting Hepatitis C Virus NS5a with ab13833.

Amounts of Hepatitis C Virus NS5a were compared to Core after spread of infection to >80% of Huh7.5 cells transfected with H77C(1a) and P346A/P351A/P354A, delta414-428 or "delta414-428+20aa" mutants.

All lanes:

Western blot - Anti-Hepatitis C Virus NS5a antibody [H26] (ab13833)

false

Image from Scheel TK et al., PLoS Pathog. 2012 May;8(5):e1002696. Epub 2012 May 24.; Fig 6.; doi:10.1371/journal.ppat.1002696; May 24, 2012, PLoS Pathog 8(5): e1002696.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

H26

亚型

IgG1

不含载体蛋白

No

反应种属

Hepatitis C virus

应用

ICC/IF, WB, ELISA

applications

特异性

Hepatitis C Virus antigen NS5a. This antibody works with the HCV genotypes 1a, 1b, 3 and 4, but not with genotype 2a.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis C virus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
存储溶液
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis C Virus NS5a (HCV NS5a) protein often simply referred to as NS5a protein is a non-structural protein important in the hepatitis C virus lifecycle. This phosphoprotein weighs approximately 56 kilodaltons and shows its expression primarily in hepatocytes cells in the liver. Researchers target NS5a as a pivotal modulator in the replication of HCV RNA and its role has made it a main target in antiviral drug development. Renowned for participating in RNA binding and interaction with other viral and host proteins NS5a supports the virus's ability to produce infectious particles making it a focus of antiviral research.
Biological function summary

NS5a plays a multifactorial role acting as part of a larger replication complex involved in HCV replication. It interacts with cellular membranes creating a replication platform for the viral RNA synthesis. Beyond its mechanical functions NS5a influences cellular pathways pivotal in the virus's persistence by modulating host cell signaling lipid metabolism and the immune response. There is active research into how NS5a manipulates host cell responses to establish chronic infection a characteristic aspect of HCV.

Pathways

NS5a significantly influences viral replication and assembly pathways. It interacts with host proteins such as cyclophilin A which regulates replication and lipid droplets important for virus assembly. The protein is integral to the PI3K-Akt pathway which NS5a manipulates to support enhanced viral RNA replication. Through its interactions NS5a links with other HCV proteins like NS3 and NS4a modulating both viral and host processes to facilitate hepatitis C virus survival and replication.

NS5a holds a direct relationship with chronic Hepatitis C infection leading to liver cirrhosis and hepatocellular carcinoma (HCC). Its ability to evade the host immune response and promote viral persistence makes it pivotal in disease progression. In the context of HCC NS5a influences the expression and activity of cellular proteins like p53 critical in cell cycle regulation. Research targets NS5a in therapeutic strategies to halt disease progression and improve patient outcomes in HCV-related liver disease.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information Hepatitis C Virus NS5a
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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33649237

2021

N6-methyladenosine modification of HCV RNA genome regulates cap-independent IRES-mediated translation via YTHDC2 recognition.

Applications

Unspecified application

Species

Unspecified reactive species

Geon-Woo Kim,Aleem Siddiqui

Journal of visualized experiments : JoVE : PubMed33191939

2020

Single-cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Raven Shah,Shuiyun Lan,Maritza N Puray-Chavez,Dandan Liu,Philip R Tedbury,Stefan G Sarafianos

PloS one 15:e0239153 PubMed32941510

2020

Reticulon-3 modulates the incorporation of replication competent hepatitis C virus molecules for release inside infectious exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Li,Ebtisam Abosmaha,Carla S Coffin,Patrick Labonté,Terence Ndonyi Bukong

Scientific reports 10:15290 PubMed32943718

2020

Hepatitis C virus enhances Rubicon expression, leading to autophagy inhibition and intracellular innate immune activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuto Shiode,Hayato Hikita,Satoshi Tanaka,Kumiko Shirai,Akira Doi,Sadatsugu Sakane,Yugo Kai,Tasuku Nakabori,Ryoko Yamada,Takahiro Kodama,Ryohei Narumi,Ryotaro Sakamori,Hidetoshi Eguchi,Takeshi Tomonaga,Tomohide Tatsumi,Tetsuo Takehara

The Journal of biological chemistry 294:7388-7402 PubMed30862675

2019

Phosphorylated tyrosine 93 of hepatitis C virus nonstructural protein 5A is essential for interaction with host c-Src and efficient viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Klinker,Sabine Stindt,Lothar Gremer,Johannes G Bode,Christoph G W Gertzen,Holger Gohlke,Oliver H Weiergräber,Silke Hoffmann,Dieter Willbold

International journal of molecular medicine 42:957-965 PubMed29786754

2018

Regulation of interferon signaling and HCV‑RNA replication by extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Kuwashiro,Shinji Iwane,Xia Jinghe,Sachiko Matsuhashi,Yuichiro Eguchi,Keizo Anzai,Kazuma Fujimoto,Toshihiko Mizuta,Naoya Sakamoto,Masanori Ikeda,Nobuyuki Kato,Iwata Ozaki

Scientific reports 7:5816 PubMed28725041

2017

A screen for novel hepatitis C virus RdRp inhibitor identifies a broad-spectrum antiviral compound.

Applications

Unspecified application

Species

Unspecified reactive species

Abhilasha Madhvi,Smita Hingane,Rajpal Srivastav,Nishant Joshi,Chandru Subramani,Rajagopalan Muthumohan,Renu Khasa,Shweta Varshney,Manjula Kalia,Sudhanshu Vrati,Milan Surjit,C T Ranjith-Kumar

Oncotarget 8:56228-56242 PubMed28915586

2017

Hepatitis C virus core protein targets 4E-BP1 expression and phosphorylation and potentiates Myc-induced liver carcinogenesis in transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cosette Abdallah,Charlène Lejamtel,Nassima Benzoubir,Serena Battaglia,Nazha Sidahmed-Adrar,Christophe Desterke,Matthieu Lemasson,Arielle R Rosenberg,Didier Samuel,Christian Bréchot,Delphine Pflieger,François Le Naour,Marie-Françoise Bourgeade

Molecular & cellular proteomics : MCP 13:3040-8 PubMed25044019

2014

A novel immuno-competitive capture mass spectrometry strategy for protein-protein interaction profiling reveals that LATS kinases regulate HCV replication through NS5A phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Hélène Meistermann,Junjun Gao,Sabrina Golling,Jens Lamerz,Sophie Le Pogam,Manuel Tzouros,Sailaja Sankabathula,Lore Gruenbaum,Isabel Nájera,Hanno Langen,Klaus Klumpp,Angélique Augustin

PLoS pathogens 8:e1002696 PubMed22654662

2012

Analysis of functional differences between hepatitis C virus NS5A of genotypes 1-7 in infectious cell culture systems.

Applications

WB

Species

Hepatitis C virus

Troels K H Scheel,Jannick Prentoe,Thomas H R Carlsen,Lotte S Mikkelsen,Judith M Gottwein,Jens Bukh
View all publications
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