抗丙型肝炎病毒NS3抗体(ab21124)
Key features and details
- Goat polyclonal to Hepatitis C Virus NS3
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Hepatitis C virus
- Isotype: IgG
概述
-
产品名称
抗丙型肝炎病毒NS3抗体
参阅全部 Hepatitis C Virus NS3 一抗 -
描述
山羊多克隆抗体to丙型肝炎病毒NS3 -
宿主
Goat -
经测试应用
适用于: ELISA, ICC/IF, WBmore details -
种属反应性
与反应: Hepatitis C virus -
免疫原
Recombinant full length protein (genotype 1a).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
纯化说明
Sodium sulfate precipitation & ion-exchange chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21124于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent dilution.
|
|
| ICC/IF |
Use at an assay dependent dilution.
|
|
| WB |
Use at an assay dependent dilution. Predicted molecular weight: 72 kDa.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent dilution. |
|
ICC/IF
Use at an assay dependent dilution. |
|
WB
Use at an assay dependent dilution. Predicted molecular weight: 72 kDa. |
靶标
-
相关性
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa. -
细胞定位
Endoplasmic reticulum membrane -
数据库链接
- SwissProt: P26663 Hepatitis C virus
- SwissProt: P26664 Hepatitis C virus
- SwissProt: Q81258 Hepatitis C virus
-
别名
- Hepatitis C Virus NS3 antibody
- NS3 antibody
- NS3P antibody
see all
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab21124 被引用在 6 文献中.
- Chen KY et al. A highly sensitive cell-based luciferase assay for high-throughput automated screening of SARS-CoV-2 nsp5/3CLpro inhibitors. bioRxiv N/A:N/A (2021). PubMed: 34981051
- Wozniak AL et al. Hepatitis C virus promotes virion secretion through cleavage of the Rab7 adaptor protein RILP. Proc Natl Acad Sci U S A 113:12484-12489 (2016). PubMed: 27791088
- Quan H et al. Hepatitis C virus core protein epigenetically silences SFRP1 and enhances HCC aggressiveness by inducing epithelial-mesenchymal transition. Oncogene N/A:N/A (2013). PubMed: 23770846
- Kannan RP et al. Hepatitis C virus infection causes cell cycle arrest at the level of initiation of mitosis. J Virol 85:7989-8001 (2011). WB . PubMed: 21680513
- McGivern DR et al. Impaired replication of hepatitis C virus containing mutations in a conserved NS5B retinoblastoma protein-binding motif. J Virol 83:7422-33 (2009). WB . PubMed: 19458004
- Wang T et al. Role of Hepatitis C virus core protein in viral-induced mitochondrial dysfunction. J Viral Hepat : (2009). WB ; Hepatitis C virus . PubMed: 20002299