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抗丙型肝炎病毒E2抗体(ab54556)

Submit a review Q&A (4)

Key features and details

  • Rabbit polyclonal to Hepatitis C Virus E2
  • Suitable for: WB, ELISA
  • Reacts with: Hepatitis C virus
  • Isotype: IgG

概述

  • 产品名称

    抗丙型肝炎病毒E2抗体
    参阅全部 Hepatitis C Virus E2 一抗

  • 描述

    兔多克隆抗体to丙型肝炎病毒E2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ELISAmore details

  • 种属反应性

    与反应: Hepatitis C virus

  • 免疫原

    Synthetic peptide corresponding to Hepatitis C Virus E2 (N terminal).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab54556于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 µg/ml. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA
Use at an assay dependent dilution.
说明
WB
Use a concentration of 1 µg/ml. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA
Use at an assay dependent dilution.

靶标

  • 相关性

    Hepatitis C E2 is a virus envelope glycoprotein which forms a heterodimer with the E1 protein. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.

  • 细胞定位

    Viral envelope protein.

  • 数据库链接

    • 别名

      • Core protein antibody
      • E1 protein antibody
      • E2 protein antibody
      • Envelope glycoprotein E2 antibody
      • gp68 antibody
      • gp70 antibody
      • HCV E2 antibody
      • NS1 antibody
      • NS2 protein antibody
      • NS3 protease/helicase antibody
      • NS4A protein antibody
      • NS4B protein antibody
      • NS5A protein antibody
      • NS5B RNA dependent RNA polymerase antibody
      • P7 protein antibody
      • Polyprotein antibody
      see all

    文献 (0)

    发表研究结果有使用 ab54556？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab54556 尚未被引用在任何文献中。

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Question

    Dear Mr or Ms,



    1- Has that antibody been tested against E2 protein from HCV genotype 1A?



    2- Does it have the ability to recognize E2 protein from HCV genotype 1A?

    Read More
    Answer

    Thank you for contacting us.





    This antibody hs been tested against E2 protein from HCV genotype 1A. It works well



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Good afternoon !

    I have a question concerning Anti-Hepatitis C Virus E2 antibody (ab54556): have you verified their ability to recognize protein E2 from genotype 1B of HCV?

    I would also like to know what is the exact immunogen You used to generate this antibodies (to which aminoacids from E2 sequence it corresponds).

    Thank You in advance for your answer.

    Read More
    Answer

    Thank you for contacting us and your interest in our products.

    The immunogen sequence used to raise the anti-Hepatitis C Virus E2 antibody (ab54556) is proprietary information. However, in order to aid your research I am able to share it with you. Please do not share or publish this information. It is strictly for your own use.

    I can confirm that this antibody was raised against Hepatitis C Virus, genotype: 1B. It's immunogen corresponds to amino acids xxxxxx of Hepatitis C Virus E2.

    I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

    Read More

    Question

    Hello,I am looking for antibodies (preferentially mouseMAb so I can use same secondary), reacting withvirions of hepatitis A virus (HAV), hepatitis C virus (HCV) and parvovirus B19. I have selected ab64295 forB19 and ab8254 for HAV. Could you, please confirm these would detect the intactvirions of B19 and HAV? Also, I am not sure which of your anti-HCV antibodies to choose for this purpose. Could you, please advice?

    Read More
    Answer

    Thank you for contacting us.

    Following your enquiry I have checked all our HAV, HCV and Parvovirus B19 antibodies and doublechecked with the originators of these if the antibodies would detect intact virions. Thank you for your patience while waiting for the response.

    Concerning Parvovirus B19 antibody ab64295:
    Indeed, we can confirm that this antibody does detect the intact virus particles. This monoclonal antibody is specific for an epitope common to VP1 and VP2 structural proteins of human Parvovirus B19. These proteins do not need to be purified or the virus does not need to be disrupted in order for the antibody to recognize the target(s).

    Concerning Anti-Hepatitis A Virus antibody ab8254:
    The Hepatitis A pHM-175 strain was used as the immunogen. The clone MK-01 recognises hepatitis A virus surface antigen, unfortunately no further details are available if this antibody also detects intact virions. However, I am happy to let you know that we have ab1018 available which detects intact Hepatitis A virus:
    https://www.abcam.com/Hepatitis-A-Virus-antibody-ab1018.html
    https://www.abcam.com/Hepatitis-A-Virus-antibody-ab1018.html.

    Concerning Anti-Hepatitis C Virus antibodies: As the envelope glycoproteins, E1 and E2, are essential components of the HCV virion, I have looked for our antibodies against these targets.
    ab54555 and ab54556: Both antibodies can detect intact Hepatitis C virions. This has so far only been tested for the inactivated form, unfortunately not yet for non-inactivated virions.
    ab20044, ab20852, ab21306 : The target protein recognized by each antibody (HCV E1 or E2) are identified by testing on Western blot. These antibodies have also been tested by ELISA and IF/IHC, but these test methods do not identify if the epitopes are recognized on intact virions. It is therefore not known if these antibodies will recognize intact HCV virions.
    https://www.abcam.com/Hepatitis-C-Virus-E1-antibody-ab54555.html https://www.abcam.com/Hepatitis-C-Virus-E1-antibody-ab54555.html.
    https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-ab54556.html https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-ab54556.html.
    https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-ab20044.html https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-ab20044.html.
    https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-BDI167-ab20852.html https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-BDI167-ab20852.html.
    https://www.abcam.com/Hepatitis-C-Virus-E1-antibody-BDI198-ab21306.html https://www.abcam.com/Hepatitis-C-Virus-E1-antibody-BDI198-ab21306.html.

    Please be reassured that all our products are covered by our Abpromise: We will happily offer Scientific Support in the event that a product is not functioning in the applications and species cited on the product datasheet (and the problem has been reported within 6 months of purchase). If it appears that the antibody is faulty, a replacement, credit or refund will be offered (www.abcam.com/Abpromise).

    All tested applications are specified on our datasheets, which are updated as soon as any new information is brought to our attention. If you decide to go ahead and purchase one of these products, please contact our Scientific Support team prior to the purchase as you might be eligible for our testing discount program:

    As we do not currently have images for all these antibodies on our datasheets, I can offer a discount off a future purchase if you buy one or more of the products now, use it a tested application and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each.The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    Read More

    Question

    Good morning, I would like to know if products #ab54555 and #ab54556 which are rabbit polyclonal antibodies produced against E1 protein from Hepatitis C Virus are suitable to use with Genotype 2A. Regards,

    Read More
    Answer

    Thank you for your inquiry. I can confirm that these antibodies are raised against Hepatitis C Virus, genotype: 1B. They were never testedwith genotype 2A and might therefore not be suitable for genotype 2A. I am sorry that I could not provide you with an positive answer on this occasion and hope this information is nevertheless helpful.

    Read More

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