抗乙型肝炎病毒X antigen抗体[3F6-G10] (ab235)
Key features and details
- Mouse monoclonal [3F6-G10] to Hepatitis B Virus X antigen
- Suitable for: ELISA, IHC-P, IHC-Fr
- Reacts with: Hepatitis B virus
- Isotype: IgG2a
概述
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产品名称
抗乙型肝炎病毒X antigen抗体[3F6-G10]
参阅全部 Hepatitis B Virus X antigen 一抗 -
描述
小鼠单克隆抗体[3F6-G10] to乙型肝炎病毒X antigen -
宿主
Mouse -
特异性
HB-X antigen (17 kD). We have received mixed reports regarding how ab235 works in Western blot, and we are not able to guarantee this antibody in WB. -
经测试应用
适用于: ELISA, IHC-P, IHC-Frmore details -
种属反应性
与反应: Hepatitis B virus -
免疫原
Fusion protein corresponding to Hepatitis B Virus X antigen. HB-Xag-Protein A Fusion protein. Spleen cells from immunised BALB/c mice were fused with cells of the Sp-2/0-Ag14 mouse myeloma cell line.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified IgG prepared by affinity chromotography on protein G from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
3F6-G10 -
骨髓瘤
unknown -
同种型
IgG2a -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
1/100.
This antibody may not require protein digestion pre-treatment or antigen retrieval on paraffin sections. |
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IHC-Fr |
1/100.
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说明 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
1/100. This antibody may not require protein digestion pre-treatment or antigen retrieval on paraffin sections. |
IHC-Fr
1/100. |
靶标
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相关性
Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC. -
数据库链接
- SwissProt: O91531 Hepatitis B virus
- SwissProt: Q4R1S1 Hepatitis B virus
- SwissProt: Q91C38 Hepatitis B virus
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别名
- HBV X antigen antibody
- HBx antibody
- HBxAg antibody
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab235 被引用在 14 文献中.
- Guan H et al. DNA methyltransferase 1 knockdown reverses PTEN and VDR by mediating demethylation of promoter and protects against renal injuries in hepatitis B virus-associated glomerulonephritis. Cell Biosci 12:98 (2022). PubMed: 35765066
- Wan T et al. NEDD4 Induces K48-Linked Degradative Ubiquitination of Hepatitis B Virus X Protein and Inhibits HBV-Associated HCC Progression. Front Oncol 11:625169 (2021). PubMed: 33767993
- Hamamoto S et al. Enhancement of chondrogenic differentiation supplemented by a novel small compound for chondrocyte-based tissue engineering. J Exp Orthop 7:10 (2020). PubMed: 32146609
- Xie WH et al. Hepatitis B virus X protein promotes liver cell pyroptosis under oxidative stress through NLRP3 inflammasome activation. Inflamm Res 69:683-696 (2020). PubMed: 32347316
- Song C et al. Associations Between Hepatitis B Virus Infection and Risk of All Cancer Types. JAMA Netw Open 2:e195718 (2019). PubMed: 31199446