抗乙型肝炎病毒pre S2 Antigen抗体[S26] (ab8635)
![Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)](https://www.abcam.cn/ps/products/8/ab8635/Images/ab8635-15077-anti-hepatitis-b-virus-pre-s2-antigen-antibody-s26-western-blot.jpg "Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)")
Key features and details
- Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen
- Suitable for: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WB
- Reacts with: Hepatitis B virus
- Isotype: IgG1
概述
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产品名称
抗乙型肝炎病毒pre S2 Antigen抗体[S26] -
描述
小鼠单克隆抗体[S26] to乙型肝炎病毒pre S2 Antigen -
宿主
Mouse -
特异性
This antibody reacts with HBV Large and Middle Surface Antigen but not small forms (both glycosylated and non glycosylated species). -
经测试应用
适用于: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WBmore details -
种属反应性
与反应: Hepatitis B virus -
免疫原
Tissue, cells or virus corresponding to Hepatitis B virus Hepatitis B Virus pre S2 Antigen. Purified serum HBV surface antigen
Database link: A0FDJ9 -
表位
aa positions 131-139 (adw nomenclature) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
克隆
单克隆 -
克隆编号
S26 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-FoFr |
1/100.
|
|
| IHC-Fr |
1/100.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| IHC-P |
1/100.
|
|
| WB | (2) |
1/1000.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-FoFr
1/100. |
|
IHC-Fr
1/100. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
IHC-P
1/100. |
|
WB
1/1000. |
靶标
-
相关性
The human hepatitis B virus (HBV) is a DNA virus that causes chronic infections that frequently lead to the development of cirrhosis and cellular hepatocellular carcinoma. The HBsAg (surface antigen) gene is one long open reading frame but contains three in frame "start" (ATG) codons that divide the gene into three sections, pre-S1, pre-S2, and S. Because of the multiple start codons, polypeptides of three different sizes called large, middle, and small (pre-S1 + pre-S2 + S, pre-S2 + S, or S) are produced. The preS2 domain is the minimal functional unit of transcription activators that are encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC). Pre-S2 is a diagnostically important surface antigen of human hepatitis B virus. The PreS domain (S1 + S2) of Hepatitis B virus (HBV) surface antigen may be a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes. -
细胞定位
Virion membrane (By similarity). -
数据库链接
- Entrez Gene: 2828293 Hepatitis B virus
- SwissProt: A0FDJ9 Hepatitis B virus
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别名
- HBV pre S2 Antigen antibody
- HBV pre S2 protein antibody
- Hepatitis B Virus pre S2 protein antibody
see all
图片
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Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)Image courtesy of Alina Macovei by Abreview.All lanes : Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from HEK cells
Lanes 2-3 : Whole cell lysate prepared from HEK cells transfected with pCI M
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Sheep anti-mouse HRP conjugated polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 33,36,39 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
实验方案
数据表及文件
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Datasheet download
文献 (7)
ab8635 被引用在 7 文献中.
- Cui S et al. NDP52 mediates an antiviral response to hepatitis B virus infection through Rab9-dependent lysosomal degradation pathway. Nat Commun 14:8440 (2023). PubMed: 38114531
- Li W et al. Hepatitis B and Hepatitis C Virus Infection Promote Liver Fibrogenesis through a TGF-β1-Induced OCT4/Nanog Pathway. J Immunol 208:672-684 (2022). PubMed: 35022275
- Ma A et al. A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B. Hum Vaccin Immunother 16:756-778 (2020). PubMed: 31687879
- Li YX et al. Hepatitis B Virus Middle Protein Enhances IL-6 Production via p38 MAPK/NF-?B Pathways in an ER Stress-Dependent Manner. PLoS One 11:e0159089 (2016). PubMed: 27434097
- Draz MS et al. Hybrid nanocluster plasmonic resonator for immunological detection of hepatitis B virus. ACS Nano 6:7634-43 (2012). PubMed: 22934963
- Villet S et al. In vitro characterization of viral fitness of therapy-resistant hepatitis B variants. Gastroenterology 136:168-176.e2 (2009). ICC/IF . PubMed: 18996386
- Glebe D et al. Pre-s1 antigen-dependent infection of Tupaia hepatocyte cultures with human hepatitis B virus. J Virol 77:9511-21 (2003). PubMed: 12915565
