Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-Heparan Sulfate Proteoglycan 2/Perlecan抗体[EPR22844-20] - BSA and Azide free (Detector) (ab274439)

Submit a review Submit a question
Sandwich ELISA - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-20] - BSA and Azide free (Detector) (ab274439)
  • Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-20] - BSA and Azide free (Detector) (ab274439)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22844-20] to Heparan Sulfate Proteoglycan 2/Perlecan - BSA and Azide free (Detector)
  • Suitable for: Sandwich ELISA
  • Reacts with: Human

概述

  • 产品名称

    Anti-Heparan Sulfate Proteoglycan 2/Perlecan抗体[EPR22844-20] - BSA and Azide free (Detector)
    参阅全部 Heparan Sulfate Proteoglycan 2/Perlecan 一抗

  • 描述

    兔单克隆抗体[EPR22844-20] to Heparan Sulfate Proteoglycan 2/Perlecan - BSA and Azide free (Detector)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Sandwich ELISAmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    ab274439 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human HSPG. The recommended pair for sandwich ELISA is:
         Capture: ab274438, Human HSPG (Perlecan) Capture Antibody (unconjugated)
         Detector: ab274439, Human HSPG (Perlecan) Detector Antibody (unconjugated)
    The reference range value is 109.375 - 7000 pg/ml.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab274439于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR22844-27] to Heparan Sulfate Proteoglycan 2/Perlecan - BSA and Azide free (Capture) (ab274438).
说明
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR22844-27] to Heparan Sulfate Proteoglycan 2/Perlecan - BSA and Azide free (Capture) (ab274438).

靶标

  • 功能

    Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
    Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
    The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity.

  • 组织特异性

    Found in the basement membranes.

  • 疾病相关

    Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
    Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.

  • 序列相似性

    Contains 4 EGF-like domains.
    Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 11 laminin EGF-like domains.
    Contains 3 laminin G-like domains.
    Contains 3 laminin IV type A domains.
    Contains 4 LDL-receptor class A domains.
    Contains 1 SEA domain.

  • 翻译后修饰

    Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
    N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation.

  • 细胞定位

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Basement membrane specific heparan sulfate proteoglycan core protein antibody
      • Endorepellin (domain V region) antibody
      • Heparan sulfate proteoglycan of basement membrane antibody
      • HSPG 2 antibody
      • HSPG antibody
      • Hspg2 antibody
      • LG3 peptide antibody
      • Perlecan antibody
      • PGBM_HUMAN antibody
      • PLC antibody
      • Schwartz Jampel syndrome 1 (chondrodystrophic myotonia) antibody
      • SJA antibody
      • SJS antibody
      • SJS1 antibody
      see all

    图片

    • Sandwich ELISA - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-20] - BSA and Azide free (Detector) (ab274439)
      Sandwich ELISA - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-20] - BSA and Azide free (Detector) (ab274439)
      Representative standard curve from corresponding SimpleStep ELISA® Kit (ab274393).
    • Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-20] - BSA and Azide free (Detector) (ab274439)
      Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-20] - BSA and Azide free (Detector) (ab274439)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab274439？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab274439 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab274439.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com