AB318285

重组Anti-Heparan Sulfate Proteoglycan 2/Perlecan抗体[RM2059] - BSA and Azide free

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free

BOND RX™ Validated
Recombinant
RabMAb
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Rabbit Recombinant Multiclonal Heparan Sulfate Proteoglycan 2/Perlecan antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, IP, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

查看别名

Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, Perlecan, PLC, HSPG2, Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, Perlecan, Hspg2

12 Images

Immunocytochemistry/ Immunofluorescence - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling Heparan Sulfate Proteoglycan 2/Perlecan with ab318284 at 1/1000 (0.486 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing membranous and cytoplasmic staining in HUVEC cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression : SH-SY5Y.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Heparan Sulfate Proteoglycan 2/Perlecan with ab318284 at 1/1000 (0.486 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon.
The section was incubated with ab318284 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colorectal carcinoma tissue labeling Heparan Sulfate Proteoglycan 2/Perlecan with ab318284 at 1/1000 (0.486 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colorectal carcinoma.
The section was incubated with ab318284 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Heparan Sulfate Proteoglycan 2/Perlecan with ab318284 at 1/1000 (0.486 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on basement membrane of human kidney.
The section was incubated with ab318284 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell) (Left) / HUVEC (human umbilical vein endothelial cell) (Right) cells labelling Heparan Sulfate Proteoglycan 2/Perlecan with ab318284 at 1/500 dilution (0.1ug) / Magenta (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : SH-SY5Y (PMID : 25883214).

Supplier Data

Immunoprecipitation - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Heparan Sulfate Proteoglycan 2/Perlecan was immunoprecipitated from 0.35 mg EA.hy926 (human somatic cell hybrid endothelial cell) whole cell lysate with ab318284 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318284 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lane 1 : EA.hy926 (human somatic cell hybrid endothelial cell) whole cell lysate
Lane 2 : ab318284 IP in EA.hy926 (human somatic cell hybrid endothelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318284 in EA.hy926 whole cell lysate

All lanes:

Immunoprecipitation - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] (<a href='/products/primary-antibodies/heparan-sulfate-proteoglycan-2-perlecan-antibody-rm2059-ab318284'>ab318284</a>) at 1/30 dilution

All lanes:

EA.hy926 (human somatic cell hybrid endothelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 76s

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling Heparan Sulfate Proteoglycan 2/Perlecan with ab318284 at 1/1000 (0.486 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic and membranous staining in MEF cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression : Neuro-2a.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) (Left) / MEF (mouse embryo fibroblast) (Right) cells labelling Heparan Sulfate Proteoglycan 2/Perlecan with ab318284 at 1/500 dilution (0.1ug) / Magenta (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : Neuro-2a

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Heparan Sulfate Proteoglycan 2/Perlecan with ab318284 at 1/1000 (0.486 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on basement membrane of rat kidney (PMID : 24035513).
The section was incubated with ab318284 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Heparan Sulfate Proteoglycan 2/Perlecan with ab318284 at 1/1000 (0.486 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on basement membrane of mouse kidney (PMID : 23757342).
The section was incubated with ab318284 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Western blot - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Low expression : SH-SY5Y (PMID : 25883214).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] (<a href='/products/primary-antibodies/heparan-sulfate-proteoglycan-2-perlecan-antibody-rm2059-ab318284'>ab318284</a>) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

EA.hy926 (human somatic cell hybrid endothelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 469 kDa,124 kDa

false

Exposure time: 37s

Supplier Data

Western blot - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

This data was developed using ab318284, the same antibody clone in a different buffer formulation.

Low expression : RAW 264.7, J774A.1(PMID : 35887248), Neuro-2a.

The bands beneath the target band (469 kDa) are likely to be degraded target fragments.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Lane 1:

MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 5:

J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 469 kDa,124 kDa

true

Exposure time: 169s

不同偶联物与剂型 (1)

Unconjugated

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059]

关键信息

宿主种属

Rabbit

克隆

Multiclonal

克隆号

RM2059

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse, Rat

应用

IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Unsuitable for rat WB.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab318285 is the carrier-free version of ab318284.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Heparan Sulfate Proteoglycan 2 also known as Perlecan is a large multidomain proteoglycan with a mass of over 500 kDa. It contains heparan sulfate chains that contribute to its role in the extracellular matrix. Perlecan is expressed in various tissues including cartilage basement membranes and vascular tissues. It interacts with other matrix proteins providing structural integrity and influencing cell behavior.

Biological function summary

Perlecan plays a critical role in cell adhesion proliferation and growth factor signaling. It is an important component of basement membranes and forms a structural complex with other matrix proteins. Its interaction with heparan sulfate chains enables binding with various ligands such as growth factors and cytokines optimizing their availability and functionality at the cell surface. This interaction is essential in tissue maintenance and repair processes.

Pathways

Perlecan participates in the regulation of the Wnt and Hedgehog signaling pathways. These pathways influence key developmental processes and dictate cellular differentiation and migration. Perlecan and its heparan sulfate chains modulate protein interactions within these pathways notably affecting proteins such as Dsh and Ctnnb1 which are essential in signal transduction.

Perlecan involvement has been linked with tumorigenesis and cardiovascular diseases. Aberrations in its structure or expression can alter cellular microenvironments impacting key processes like angiogenesis and metastasis. Furthermore its interaction with growth factors can facilitate the progression of diseases like cancer highlighting its connection with proteins such as VEGF and TGF-beta which play major roles in disease modulation.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development (PubMed : 12435733, PubMed : 15591058, PubMed : 19789387). In muscle, it is essential for localizing acetylcholinesterase (AChE) at the neuromuscular junctions (NMJ), most probably acting as an adapter that links the acetylcholinesterase collagenic tail peptide (COLQ) to alpha-dystroglycan, and is therefore involved in the down-regulation of colinergic synaptic transmission (By similarity).. Endorepellin. Anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.. LG3 peptide. Has anti-angiogenic properties that require binding of calcium ions for full activity.

See full target information Basement membrane-specific heparan sulfate proteoglycan core protein

其他靶点

Hspg2

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Heparan Sulfate Proteoglycan 2/Perlecan抗体[RM2059] - BSA and Azide free

Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Flow Cytometry (Intracellular) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Immunoprecipitation - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Immunocytochemistry/ Immunofluorescence - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Flow Cytometry (Intracellular) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Western blot - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

Western blot - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [RM2059] - BSA and Azide free (AB318285)

不同偶联物与剂型 (1)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性

搭配使用产品

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

其他靶点

Abcam Product Promise