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重组RabMAb

重组Anti-Hemopexin抗体[EPR5610] (ab124935)

Reviews (2) Submit a question References (3)
Western blot - Anti-Hemopexin antibody [EPR5610] (ab124935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hemopexin antibody [EPR5610] (ab124935)
  • Flow Cytometry (Intracellular) - Anti-Hemopexin antibody [EPR5610] (ab124935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)
  • OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] (ab124935)
  • Anti-Hemopexin antibody [EPR5610] (ab124935)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5610] to Hemopexin
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP

概述

  • 产品名称

    Anti-Hemopexin抗体[EPR5610]
    参阅全部 Hemopexin 一抗

  • 描述

    兔单克隆抗体[EPR5610] to Hemopexin

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human Hemopexin aa 400-500. The exact sequence is proprietary.

  • 阳性对照

    • WB: Human fetal liver and plasma lysates. IHC-P: Human liver tissue. Flow Cyt (intra): HepG2 cells. ICC: HepG2 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124935于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/200.
WB (1)
1/10000 - 1/50000. Detects a band of approximately 70-75 kDa (predicted molecular weight: 52 kDa).
IP
1/10 - 1/100.
IHC-P (1)
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
说明
Flow Cyt (Intra)
1/200.
WB
1/10000 - 1/50000. Detects a band of approximately 70-75 kDa (predicted molecular weight: 52 kDa).
IP
1/10 - 1/100.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.

靶标

  • 功能

    Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

  • 组织特异性

    Expressed by the liver and secreted in plasma.

  • 序列相似性

    Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.

  • 翻译后修饰

    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Beta 1B glycoprotein antibody
      • Beta-1B-glycoprotein antibody
      • FLJ56652 antibody
      • HEMO antibody
      • HEMO_HUMAN antibody
      • Hemopexin antibody
      • Hpx antibody
      • HX antibody
      see all

    图片

    • Western blot - Anti-Hemopexin antibody [EPR5610] (ab124935)
      Western blot - Anti-Hemopexin antibody [EPR5610] (ab124935)
      All lanes : Anti-Hemopexin antibody [EPR5610] (ab124935) at 1/10000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 70-75 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)This image is courtesy of an Abreview submitted by Steffen Rickelt

      ab124935 staining Hemopexin in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Hemopexin antibody [EPR5610] (ab124935)
      Immunocytochemistry/ Immunofluorescence - Anti-Hemopexin antibody [EPR5610] (ab124935)
      Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Hemopexin with purified ab124935 at 1/200 dilution (10 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.8 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.
    • Flow Cytometry (Intracellular) - Anti-Hemopexin antibody [EPR5610] (ab124935)
      Flow Cytometry (Intracellular) - Anti-Hemopexin antibody [EPR5610] (ab124935)

      Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hemopexin (red) with ab124935 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemopexin antibody [EPR5610] (ab124935)

      ab124935, at 1/250 dilution, staining Hemopexin in paraffin-embedded Human liver tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] (ab124935)
      OI-RD Scanning - Anti-Hemopexin antibody [EPR5610] (ab124935)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Hemopexin antibody [EPR5610] (ab124935)
      Anti-Hemopexin antibody [EPR5610] (ab124935)

    文献 (3)

    发表研究结果有使用 ab124935？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab124935 被引用在 3 文献中.

    • Li X  et al. Proteomic profiling identifies signatures associated with progression of precancerous gastric lesions and risk of early gastric cancer. EBioMedicine 74:103714 (2021). PubMed: 34818622
    • Canesin G  et al. Scavenging of Labile Heme by Hemopexin Is a Key Checkpoint in Cancer Growth and Metastases. Cell Rep 32:108181 (2020). PubMed: 32966797
    • Lim JH  et al. Novel urinary exosomal biomarkers of acute T cell-mediated rejection in kidney transplant recipients: A cross-sectional study. PLoS One 13:e0204204 (2018). PubMed: 30226858

    客户评价及客户问答

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Hemopexin antibody [EPR5610]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
    Sample
    Human Tissue sections (human liver)
    Specification
    human liver
    Permeabilization
    Yes - 0.2% Triton X-100 and PBS
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Steffen Rickelt

    Verified customer

    提交于 Mar 12 2014

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