重组Anti-Hemoglobin subunit beta + Hemoglobin subunit delta抗体[EPR8322(B)] (ab131225)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8322(B)] to Hemoglobin subunit delta + Hemoglobin subunit beta
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Hemoglobin subunit beta + Hemoglobin subunit delta抗体[EPR8322(B)] -
描述
兔单克隆抗体[EPR8322(B)] to Hemoglobin subunit delta + Hemoglobin subunit beta -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Hemoglobin subunit delta aa 1-100. The exact sequence is proprietary.
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阳性对照
- Human fetal heart, fetal liver, and red blood cell (RBC) lysates; Human endometrium and spleen tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8322(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131225于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/200 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/200 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
图片
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Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)All lanes : Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/200 dilution
Lane 1 :Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697)
Lane 2 : His-tagged human Hemoglobin subunit beta/ba1 (aa 3-147) recombinant protein
Lane 3 : His-tagged human Hemoglobin subunit delta (aa 3-147) recombinant protein
Lane 4 : His-tagged full length human Hemoglobin subunit delta recombinant protein
Lane 5 : His-tagged human Hemoglobin subunit epsilon (aa 3-147) recombinant protein
Lane 6 : His-tagged human Hemoglobin subunit gamma-2 (aa 2-147) recombinant protein
Lane 7 : His-tagged human Hemoglobin subunit gamma-1 (aa 2-147) recombinant protein
Lane 8 :Recombinant Human Hemoglobin subunit zeta protein (ab95347)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 16 kDaBlocking/Dilutiing buffer and concentration: 5% NFDM/TBST
Exposure time: 100 seconds
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Western blot - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)All lanes : Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Human red blood cell (RBC) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
Immunohistochemical analysis of paraffin embedded Human endometrium tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit beta + Hemoglobin subunit delta antibody [EPR8322(B)] (ab131225)
Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling Hemoglobin subunit delta with ab131225 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab131225 被引用在 1 文献中.
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873