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AB13248

Anti-Heme Oxygenase 1 抗体 [HO-1-1]

Anti-Heme Oxygenase 1 antibody [HO-1-1]

4

(21 Reviews)

|

(390 Publications)

Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) is a mouse monoclonal antibody detecting Heme Oxygenase 1 in Western Blot, Flow Cytometry (Intra), IHC-P, ELISA. Suitable for Cow, Dog, Human, Mouse, Rat.

- Over 320 publications
- Trusted since 2004

查看别名

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

6 Images
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
  • WB

Lab

Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)

Hek293 & HL60 presumed negative or very low expression.

Loading control GAPDH at 38kDa

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1 µg/mL

Lane 1:

Hek293 at 10 µg

Lane 2:

HL60 at 10 µg

Lane 3:

HeLa at 10 µg

Lane 4:

A549 at 10 µg

Lane 5:

Hu spleen at 10 µg

Lane 6:

Ms spleen at 10 µg

Lane 7:

Rt spleen at 10 µg

Secondary

All lanes:

IRDye® 800CW Goat anti Mouse

Predicted band size: 33 kDa

Observed band size: 32 kDa

false

Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)

ab13248 at 10μg/ml staining Heme Oxygenase 1 in human lung cancer A2 cells by flow cytometery. The left image repersents staining with isotype control antibody and the right image show staining with ab13248.

This image was generated using the ascites version of the product.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)

IHC image of Hem Oxygensae 1 staining in a section of formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13248, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

This image was generated using the ascites version of the product.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
  • WB

Unknown

Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1/250 dilution

All lanes:

Human microsome lysate

Predicted band size: 33 kDa

false

Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
  • WB

Supplier Data

Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)

This image was generated using the ascites version of the product.

Lane 1:

MW marker

Lanes 2 - 7:

Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

Lane 2:

Recombinant Rat Heme Oxygenase 1

Lane 3:

Recombinant Human Heme Oxygenase 1

Lane 4:

Recombinant Human Heme Oxygenase 2

Lane 5:

MDBK cell lysate

Lane 6:

Dog liver microsome

Lane 7:

Mouse liver microsome

Predicted band size: 33 kDa

false

Sandwich ELISA - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
  • sELISA

Unknown

Sandwich ELISA - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)

Standard Curve for Heme Oxygenase 1 (Analyte : Heme Oxygenase 1 protein (Tagged) (ab85243)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) at 5μg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1 (ab13243) at 0.5μg/ml.

This image was generated using the ascites version of the product.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

HO-1-1

亚型

IgG1

不含载体蛋白

No

反应种属

Mouse, Rat, Cow, Dog, Human

应用

WB, sELISA, IHC-P, Flow Cyt (Intra)

applications

免疫原

Synthetic Peptide within Human HMOX1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P09601

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Capture at 5 μg/ml with Rabbit polyclonal to Heme Oxygenase 1 (<a href='/products/primary-antibodies/heme-oxygenase-1-antibody-ab13243'>ab13243</a>) as Detection.</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "4 µg/mL", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

产品详情

What is this antibody validated in?
Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), ELISA in Cow, Dog, Human, Mouse, Rat samples.

What is the molecular weight of Heme Oxygenase 1?
Anti-Heme Oxygenase 1 [HO-1-1] (ab13248) specifically detects a band for Heme Oxygenase 1 (UniProt: P09601) at a molecular weight of 34.6kDa.

Trusted by the scientific community
Anti-Heme Oxygenase 1 [HO-1-1] (ab13248) was first used in a scientific publication in 2004 and has been cited over 320 times in peer-reviewed journals.

Reviewed by scientists
Anti-Heme Oxygenase 1 [HO-1-1] (ab13248) has over 15 independent reviews from customers.

性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
纯化说明
Purified from TCS.
存储溶液
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.
Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed : 11121422, PubMed : 19556236, PubMed : 7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed : 20055707).. Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
See full target information HMOX1

文献 (390)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed41008584

2025

Thymol Preserves Spermatogenesis and Androgen Production in Cisplatin-Induced Testicular Toxicity by Modulating Ferritinophagy, Oxidative Stress, and the Keap1/Nrf2/HO-1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Amira M Badr,Sheka Aloyouni,Yasmin Mahran,Hanan Henidi,Elshaymaa I Elmongy,Haya M Alsharif,Aliyah Almomen,Sahar Soliman

Korean journal of anesthesiology : PubMed40590087

2025

Human placental mesenchymal stem cell-derived exosomes carrying hsa-let-7i-5p mitigate lung injury in a murine model of aspiration pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Wei Chuang,Hong-Phuc Nguyen Vo,Yen-Hua Huang,I-Lin Tsai,Chao-Yuan Chang,Chun-Jen Huang

Frontiers in pharmacology 16:1567942 PubMed40535769

2025

Pomolic acid induces ferroptosis-mediated cell death in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbin Ji,Yanbin Zhang,Wenhao Ji,Hui Zhang,Biao Qin,Xiao-Liang Xing,Zaiqi Zhang

Journal of microbiology and biotechnology 35:e2501014 PubMed40443236

2025

Hepatoprotective Effect of Meroterpenoid-Rich Fraction from the Ethanolic Extract of on -Butyl Hydroperoxide-Induced Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sujin Lim,Ji Young Hwang,Kyoung Mi Moon,Hyeon Hak Jeong,Jaeseong Seo,Hyeung-Rak Kim,Bonggi Lee

Probiotics and antimicrobial proteins : PubMed40405038

2025

Probiotics Mixture, Prohep: a Potential Adjuvant for Low-Dose Sorafenib in Metabolic Dysfunction-Associated Steatotic Liver Disease-Associated Hepatocellular Carcinoma Suppression Through Modulating Gut Microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfei Zhang,Emily Kwun Kwan Lo,Congjia Chen,Jetty Chung-Yung Lee, Felicianna,Marsena Jasiel Ismaiah,Hoi Kit Matthew Leung,Dorothy Hin Lam Tsang,Hani El-Nezami

iScience 28:112116 PubMed40160414

2025

Dual role of HO-1 in mediating antiviral immune responses and mitigating excessive inflammatory damage during influenza virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Ma,Peng Zhang,Xingqiong Li,Baihe Sun,Yuhuan Li,Jiandong Jiang

Nutrients 17: PubMed39940407

2025

EGCG Alleviates DSS-Induced Colitis by Inhibiting Ferroptosis Through the Activation of the Nrf2-GPX4 Pathway and Enhancing Iron Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Junzhou Chen,Conghui Yin,Yilong Zhang,Xin Lai,Chen Liu,Yuheng Luo,Junqiu Luo,Jun He,Bing Yu,Quyuan Wang,Huifen Wang,Daiwen Chen,Aimin Wu

Frontiers in immunology 15:1513196 PubMed39902039

2025

Inflammatory biomarkers and therapeutic potential of milk exosome-mediated CCL7 siRNA in murine intestinal ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

WenDong Chen,WenPing Xu,Li Ma,Chun Bi,MeiXu Yang,Wei Yang

Journal of tissue engineering 15:20417314241287487 PubMed39698514

2024

Intranasal delivery of macrophage cell membrane cloaked biomimetic drug-nanoparticle system attenuates acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhao,Xin Shen,Yinqiang Fan,Ning Wei,Zijie Ling,Yinlian Yao,Shilong Fan,Jiahao Liu,Yiming Shao,Zhikun Zhou,Hua Jin

Cancers 16: PubMed39682125

2024

Casein Kinase 2 Inhibitor, CX-4945, Induces Apoptosis and Restores Blood-Brain Barrier Homeostasis in In Vitro and In Vivo Models of Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Bova,Deborah Mannino,Ayomide E Salako,Emanuela Esposito,Alessia Filippone,Sarah A Scuderi
View all publications

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