Anti-Heme Oxygenase 1 抗体 [HO-1-1]
Anti-Heme Oxygenase 1 antibody [HO-1-1]
- BOND RX™ Validated
- 了解详情
4
(21 Reviews)
|
(390 Publications)
Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) is a mouse monoclonal antibody detecting Heme Oxygenase 1 in Western Blot, Flow Cytometry (Intra), IHC-P, ELISA. Suitable for Cow, Dog, Human, Mouse, Rat.
- Over 320 publications
- Trusted since 2004
查看别名
HO, HO1, HMOX1, Heme oxygenase 1, HO-1
- WB
Lab
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
This image was generated using the ascites version of the product.
All lanes:
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1 µg/mL
Lane 1:
Hek293 at 10 µg
Lane 2:
HL60 at 10 µg
Lane 3:
HeLa at 10 µg
Lane 4:
A549 at 10 µg
Lane 5:
Hu spleen at 10 µg
Lane 6:
Ms spleen at 10 µg
Lane 7:
Rt spleen at 10 µg
Secondary
All lanes:
IRDye® 800CW Goat anti Mouse
Predicted band size: 33 kDa
Observed band size: 32 kDa
false
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
ab13248 at 10μg/ml staining Heme Oxygenase 1 in human lung cancer A2 cells by flow cytometery. The left image repersents staining with isotype control antibody and the right image show staining with ab13248.
This image was generated using the ascites version of the product.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
IHC image of Hem Oxygensae 1 staining in a section of formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13248, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This image was generated using the ascites version of the product.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
- WB
Unknown
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
This image was generated using the ascites version of the product.
All lanes:
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1/250 dilution
All lanes:
Human microsome lysate
Predicted band size: 33 kDa
false
- WB
Supplier Data
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
This image was generated using the ascites version of the product.
Lane 1:
MW marker
Lanes 2 - 7:
Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)
Lane 2:
Recombinant Rat Heme Oxygenase 1
Lane 3:
Recombinant Human Heme Oxygenase 1
Lane 4:
Recombinant Human Heme Oxygenase 2
Lane 5:
MDBK cell lysate
Lane 6:
Dog liver microsome
Lane 7:
Mouse liver microsome
Predicted band size: 33 kDa
false
- sELISA
Unknown
Sandwich ELISA - Anti-Heme Oxygenase 1 antibody [HO-1-1] (AB13248)
Standard Curve for Heme Oxygenase 1 (Analyte : Heme Oxygenase 1 protein (Tagged) (ab85243)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) at 5μg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1 (ab13243) at 0.5μg/ml.
This image was generated using the ascites version of the product.
反应性数据
产品详情
Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), ELISA in Cow, Dog, Human, Mouse, Rat samples.
What is the molecular weight of Heme Oxygenase 1?
Anti-Heme Oxygenase 1 [HO-1-1] (ab13248) specifically detects a band for Heme Oxygenase 1 (UniProt: P09601) at a molecular weight of 34.6kDa.
Trusted by the scientific community
Anti-Heme Oxygenase 1 [HO-1-1] (ab13248) was first used in a scientific publication in 2004 and has been cited over 320 times in peer-reviewed journals.
Reviewed by scientists
Anti-Heme Oxygenase 1 [HO-1-1] (ab13248) has over 15 independent reviews from customers.
性能和储存信息
形式
纯度
纯化说明
存储溶液
运输条件
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.
Pathways
Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
文献 (390)
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