Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
使用敲除细胞株进行验证重组RabMAb

重组Anti-Heme Oxygenase 1抗体[EPR1390Y] (ab68477)

Reviews (5) Submit a question References (194)
Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
  • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
  • Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
  • Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1390Y] to Heme Oxygenase 1
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE

概述

  • 产品名称

    Anti-Heme Oxygenase 1抗体[EPR1390Y]
    参阅全部 Heme Oxygenase 1 一抗

  • 描述

    兔单克隆抗体[EPR1390Y] to Heme Oxygenase 1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), ICC/IF, WB, IPmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HepG2, A549, rat kidney, rat spleen, mouse kidney cell lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab68477于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF (1)
1/100 - 1/250.
WB (4)
1/1000 - 1/20000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP
1/20.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/250.
WB
1/1000 - 1/20000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP
1/20.
应用说明
Is unsuitable for IHC-P.

靶标

  • 功能

    Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.

  • 序列相似性

    Belongs to the heme oxygenase family.

  • 细胞定位

    Microsome. Endoplasmic reticulum.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 32 kD antibody
    • bK286B10 antibody
    • D8Wsu38e antibody
    • heat shock protein 32 kD antibody
    • heat shock protein 32kD antibody
    • Heat shock protein antibody
    • Heme oxygenase (decycling) 1 antibody
    • Heme oxygenase 1 antibody
    • Hemox antibody
    • HMOX 1 antibody
    • Hmox antibody
    • Hmox1 antibody
    • HMOX1_HUMAN antibody
    • HO 1 antibody
    • HO antibody
    • HO-1 antibody
    • HO1 antibody
    • Hsp32 antibody
    see all

图片

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/10000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : HMOX1 knockout A549 cell lysate
    Lane 3 : Human Spleen tissue lysate
    Lane 4 : HL-60 cell lysate
    Lane 5 : MCF7 cell lysate
    Lane 6 : HeLa cell lysate
    Lane 7 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa



    Lanes 1 - 7: Merged signal (red and green). Green - ab68477 observed at 33 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab68477 was shown to react with Heme Oxygenase 1 in wild-type A549 cells in Western blot with loss of signal observed in HMOX1 knockout cell line ab269503 (knockout cell lysate ab269665). Wild-type A549 and HMOX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab68477 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Heme Oxygenase 1 with purified ab68477 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1: PBS only.

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/1000 dilution

    Lane 1 : Hek293
    Lane 2 : HL60
    Lane 3 : HeLa
    Lane 4 : A549
    Lane 5 : Hu spleen
    Lane 6 : Ms spleen
    Lane 7 : Rt spleen

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye® 800CW Goat anti Rabbit

    Predicted band size: 33 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?



    Hek293 & HL60 presumed negative or very low expression.

    Loading control GAPDH at 38kDa

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/1000 dilution

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates with 5% NFDM/TBST
    Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 4 : Mouse spleen lysates with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 10 seconds


    We are unsure how to define the extra bands.

  • Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

    Intracellular Flow Cytometry analysis of A549 (human lung carcinoma) cells labeling with purified ab68477 at 1/200 dilution (1ug/ml) (Red). Cells were fixed with4% paraformaldehydeand permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody.Rabbit monoclonal IgG (Black) (ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control. 

     

     

  • Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    Immunoprecipitation - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

    ab68477 (purified) at 1/20 immunoprecipitating Heme Oxygenase 1 in A549 (Human lung carcinoma cell line) whole cell lysate.

    Lane 1 (input): A549 whole cell lysate (10ug).

    Lane 2 (+): ab68477 + A549 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133267 in HeLa whole cell lysate.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/20000 dilution (purified)

    Lane 1 : Rat kidney lysate
    Lane 2 : Rat spleen lysate
    Lane 3 : Mouse kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 33 kDa
    Observed band size: 33 kDa



    Blocking and dilution buffer: 5% NFDM/TBST

  • Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)

文献 (194)

发表研究结果有使用 ab68477？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab68477 被引用在 194 文献中.

  • Romero-Miguel D  et al. N-acetylcysteine during critical neurodevelopmental periods prevents behavioral and neurochemical deficits in the Poly I:C rat model of schizophrenia. Transl Psychiatry 14:14 (2024). PubMed: 38191622
  • Li J  et al. Oxygen-carrying sequential preservation mitigates liver grafts ischemia-reperfusion injury. iScience 26:105858 (2023). PubMed: 36636350
  • Wang Y  et al. Pretreatment with rosavin attenuates PM2.5-induced lung injury in rats through antiferroptosis via PI3K/Akt/Nrf2 signaling pathway. Phytother Res 37:195-210 (2023). PubMed: 36097321
  • Stukas D  et al. Targeting AHR Increases Pancreatic Cancer Cell Sensitivity to Gemcitabine through the ELAVL1-DCK Pathway. Int J Mol Sci 24:N/A (2023). PubMed: 37685961
  • Liang Z  et al. The role of HIF-1α/HO-1 pathway in hippocampal neuronal ferroptosis in epilepsy. iScience 26:108098 (2023). PubMed: 37876811
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-Heme Oxygenase 1 antibody [EPR1390Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Skov-3 cells)
Gel Running Conditions
Non-reduced Denaturing (10)
Loading amount
60 µg
Treatment
50 µM hemin
Specification
Skov-3 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 08 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Heme Oxygenase 1 antibody [EPR1390Y]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB-231)
Permeabilization
Yes - 0.2% Triton X-100
Specification
MDA-MB-231
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 13 2021

Western blot abreview for Anti-Heme Oxygenase 1 antibody [EPR1390Y]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 13 2016

Western blot abreview for Anti-Heme Oxygenase 1 antibody [EPR1390Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS-7 cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
100000 cells
Treatment
30 nM Bortezomib for 24 hours
Specification
COS-7 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 22 2015

Western blot abreview for Anti-Heme Oxygenase 1 antibody [EPR1390Y]

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10 % gel)
Sample
Rat Tissue lysate - whole (aorta)
Specification
aorta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 22 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com