Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Heme Oxygenase 1抗体(ab82585)

Submit a review Q&A (1)References (5)
Western blot - Anti-Heme Oxygenase 1 antibody (ab82585)

    Key features and details

    • Rabbit polyclonal to Heme Oxygenase 1
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Dog, Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Heme Oxygenase 1抗体
      参阅全部 Heme Oxygenase 1 一抗

    • 描述

      兔多克隆抗体to Heme Oxygenase 1

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Mouse, Rat, Dog, Human

    • 免疫原

      Synthetic peptide from the N terminal of Human Heme Oxygenase 1

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab82585于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
    说明
    WB
    1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

    靶标

    • 功能

      Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.

    • 序列相似性

      Belongs to the heme oxygenase family.

    • 细胞定位

      Microsome. Endoplasmic reticulum.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • 32 kD antibody
      • bK286B10 antibody
      • D8Wsu38e antibody
      • heat shock protein 32 kD antibody
      • heat shock protein 32kD antibody
      • Heat shock protein antibody
      • Heme oxygenase (decycling) 1 antibody
      • Heme oxygenase 1 antibody
      • Hemox antibody
      • HMOX 1 antibody
      • Hmox antibody
      • Hmox1 antibody
      • HMOX1_HUMAN antibody
      • HO 1 antibody
      • HO antibody
      • HO-1 antibody
      • HO1 antibody
      • Hsp32 antibody
      see all

    图片

    • Western blot - Anti-Heme Oxygenase 1 antibody (ab82585)
      Western blot - Anti-Heme Oxygenase 1 antibody (ab82585)
      All lanes : Anti-Heme Oxygenase 1 antibody (ab82585) at 1/1000 dilution

      Lane 1 : Heme Oxygenase 1 Recombinant Rat Protein
      Lane 2 : Heme Oxygenase 1 Recombinant Human Protein
      Lane 3 : Heme Oxygenase 2 Recombinant Human Protein
      Lane 4 : Dog Liver Microsome Extract
      Lane 5 : Mouse Liver Microsome Extract
      Lane 6 : Rat Liver Microsome Extract
      Lane 7 : Human Liver Microsome Extract

      Developed using the ECL technique.

      Predicted band size: 33 kDa
      Observed band size: 33 kDa

    文献 (5)

    发表研究结果有使用 ab82585？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab82585 被引用在 5 文献中.

    • Shi H  et al. Susceptibility of cervical cancer to dihydroartemisinin-induced ferritinophagy-dependent ferroptosis. Front Mol Biosci 10:1156062 (2023). PubMed: 37065442
    • Uto K  et al. Hydrogen-rich solution attenuates cold ischemia-reperfusion injury in rat liver transplantation. BMC Gastroenterol 19:25 (2019). PubMed: 30736744
    • Barta T  et al. Endothelin-1-induced hypertrophic alterations and heme oxygenase-1 expression in cardiomyoblasts are counteracted by beta estradiol: in vitro and in vivo studies. Naunyn Schmiedebergs Arch Pharmacol 391:371-383 (2018). WB ; Rat . PubMed: 29354880
    • Liu X  et al. Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO-1 signaling pathway. Exp Ther Med 16:1079-1086 (2018). PubMed: 30116359
    • Wang Y  et al. Retinoprotective Effects of Bilberry Anthocyanins via Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Mechanisms in a Visible Light-Induced Retinal Degeneration Model in Pigmented Rabbits. Molecules 20:22395-410 (2015). PubMed: 26694327

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Question

    Actually, ab53942 is the only one thatI have known reacting with rabbit, I have tried some other antioxidantenzymes such as HO-1andNQO1 but they don't have reactivity with rabbit so they didn't work.We don't want to try any other new antibody in case it is not certain to react with rabbit, our material is very precious since this is an animal experiment and also our time is precious too. If you are able to homology check of your antibodies such as HO-1, NQO1, GST, GCLM, GCLCwith rabbit,may beuseful for our study. And please let me know about the exact time for replacement for ab53942 when you know about it. Thank you for your attention.

    Read More
    Answer

    I have found two antibodies for rabbit samples that may be effective for detecting two of the proteins you specified. Anti-GCLC ab80841 is raised against an immunogen sequence that is conserved 100% in the rabbit sequence listed in the UniProt entry for accession G1T442: http://www.uniprot.org/uniprot/G1T442 The immunogen sequence for ab80841 is CRWGVISASVDDRTREERG. The datasheet is at the following URL: Click here (or use the following: https://www.abcam.com/GCLC-antibody-ab80841.html). For HO-1, I found ab82585: Click here (or use the following: https://www.abcam.com/Heme-Oxygenase-1-antibody-ab82585.html). I have requested the rabbit data from the originating laboratory and will forward this when I receive it.. Both of these are rabbit polyclonals, so a secondary anti-rabbit IgG antibody will likely stain denatured and reduced endogenous rabbit IgG at 25kDa and 50kDa for the light and heavy IgG subunits, but you should still be able to clearly distinguish the signal from the protein of interest. I am looking into obtaining a different lot of ab53942 and hope to have an update for you tomorrow. In the meantime, if you have any questions, please do not histiate to ask.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com