重组Anti-HECTD2抗体[EPR11554] (ab173572)
![Western blot - Anti-HECTD2 antibody [EPR11554] (ab173572)](https://www.abcam.cn/ps/products/173/ab173572/Images/ab173572-197088-anti-hectd2-antibody-epr11554-western-blot.jpg "Western blot - Anti-HECTD2 antibody [EPR11554] (ab173572)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11554] to HECTD2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HECTD2抗体[EPR11554]
参阅全部 HECTD2 一抗 -
描述
兔单克隆抗体[EPR11554] to HECTD2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Orangutan
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免疫原
Synthetic peptide within Human HECTD2 aa 600-700 (Cysteine residue). The exact sequence is proprietary.
Database link: Q5U5R9 -
阳性对照
- MCF-7, NIH:OVCAR-3 and HeLa cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11554 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173572于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 88 kDa.
|
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 88 kDa. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. - Information by UniProt
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数据库链接
- Entrez Gene: 143279 Human
- Entrez Gene: 226098 Mouse
- Entrez Gene: 100172010 Orangutan
- Entrez Gene: 309514 Rat
- SwissProt: Q5U5R9 Human
- SwissProt: Q8CDU6 Mouse
- SwissProt: Q5RD78 Orangutan
- Unigene: 596096 Human
see all -
别名
- FLJ16050 antibody
- FLJ37306 antibody
- HECD2_HUMAN antibody
see all
图片
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Western blot - Anti-HECTD2 antibody [EPR11554] (ab173572)All lanes : Anti-HECTD2 antibody [EPR11554] (ab173572) at 1/1000 dilution
Lane 1 : MCF-7 cell lysates
Lane 2 : NIH:OVCAR-3 cell lysates
Lane 3 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 88 kDa -
![Anti-HECTD2 antibody [EPR11554] (ab173572)](https://www.abcam.cn/ps/products/173/ab173572/Images/ab173572-2-benefits-of-recombinant-antibodies.png)
Anti-HECTD2 antibody [EPR11554] (ab173572)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab173572 被引用在 1 文献中.
- Ryu TY et al. Human gut-microbiome-derived propionate coordinates proteasomal degradation via HECTD2 upregulation to target EHMT2 in colorectal cancer. ISME J 16:1205-1221 (2022). PubMed: 34972816