重组Anti-HEC1/HEC抗体[EPR5342] (ab109496)
![Western blot - Anti-HEC1/HEC antibody [EPR5342] (ab109496)](https://www.abcam.cn/ps/products/109/ab109496/Images/ab109496-122382-anti-hec1-antibody-epr5342-western-blot.jpg "Western blot - Anti-HEC1/HEC antibody [EPR5342] (ab109496)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5342] to HEC1/HEC
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HEC1/HEC抗体[EPR5342]
参阅全部 HEC1/HEC 一抗 -
描述
兔单克隆抗体[EPR5342] to HEC1/HEC -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat, HeLa, NCCIT, and Human spleen lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5342 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109496于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 74 kDa.
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| 说明 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 74 kDa. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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功能
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
序列相似性
Belongs to the NDC80/HEC1 family. -
发展阶段
Expression peaks in mitosis. -
翻译后修饰
Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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数据库链接
- Entrez Gene: 10403 Human
- Entrez Gene: 67052 Mouse
- Entrez Gene: 301701 Rat
- Omim: 607272 Human
- SwissProt: O14777 Human
- SwissProt: Q9D0F1 Mouse
- Unigene: 414407 Human
- Unigene: 225956 Mouse
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别名
- from Entrez Gene ; antibody
- HEC antibody
- Highly expressed in cancer antibody
see all
图片
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Western blot - Anti-HEC1/HEC antibody [EPR5342] (ab109496)All lanes : Anti-HEC1/HEC antibody [EPR5342] (ab109496) at 1/10000 dilution
Lane 1 : Jurkat lysate
Lane 2 : HeLa lysate
Lane 3 : NCCIT lysate
Lane 4 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 74 kDa -
![Anti-HEC1/HEC antibody [EPR5342] (ab109496)](https://www.abcam.cn/ps/products/109/ab109496/Images/ab109496-2-benefits-of-recombinant-antibodies.png)
Anti-HEC1/HEC antibody [EPR5342] (ab109496)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab109496 被引用在 1 文献中.
- Luo K et al. Integrated bioinformatics analysis and experimental validation reveals hub genes of rheumatoid arthritis. Exp Ther Med 26:480 (2023). PubMed: 37745040