Anti-HEC1/HEC抗体(ab117415)
Key features and details
- Goat polyclonal to HEC1/HEC
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-HEC1/HEC抗体
参阅全部 HEC1/HEC 一抗 -
描述
山羊多克隆抗体to HEC1/HEC -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human HEC1/HEC aa 600-700 (C terminal) (Cysteine residue).
Database link: NP_006092.1 -
阳性对照
- Human adrenal and liver tissues; HeLa cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117415于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 74 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 74 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
序列相似性
Belongs to the NDC80/HEC1 family. -
发展阶段
Expression peaks in mitosis. -
翻译后修饰
Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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数据库链接
- Entrez Gene: 10403 Human
- Omim: 607272 Human
- SwissProt: O14777 Human
- Unigene: 414407 Human
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别名
- from Entrez Gene ; antibody
- HEC antibody
- Highly expressed in cancer antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEC1/HEC antibody (ab117415)
ab117415, at 5 ug/ml staining HEC1/HEC in Formalin-fixed, Paraffin-embedded Human adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEC1/HEC antibody (ab117415)ab117415, at 5 ug/ml staining HEC1/HEC in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Western blot - Anti-HEC1/HEC antibody (ab117415)Anti-HEC1/HEC antibody (ab117415) at 0.5 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab117415 尚未被引用在任何文献中。